免疫组化和PCR检测乳腺癌Her-2、EGFRt和CK5/6过表达水平用于乳腺癌疗效的评估  

Overexpression of Her-2,EGFR and CK5/6 with immunohistochemistry and real-time quantitative PCR for breast cancer

作　　者：陈颖[1] 李睿[1] 刘源[1] 濮杰[1] 史春颖[1] 戚基萍[1] 王兵[1] 张凌[1] 

机构地区：[1]哈尔滨医科大学附属第一医院放射线科,黑龙江哈尔滨150001

出　　处：《哈尔滨医科大学学报》2013年第1期56-59,共4页Journal of Harbin Medical University

基　　金：黑龙江省教育厅科学技术研究项目(11551285)

摘　　要：目的比较免疫组化方法(IHC)和实时定量PCR(RT-PCR)对乳腺癌易感基因Her-2、EGFR、CK5/6在乳腺癌治疗和预后中的应用。方法对41例乳腺癌患者分别进行癌组织IHC和RT-PCR检测,测定癌组织Her-2、EGFR、CK5/6基因的过表达水平,分析比较两种方法检测的灵敏度和特异性。结果 41例乳腺癌患者均为女性,应用免疫组化方法,Her-2阳性表达21例,EGFR呈阳性表达8例,CK5/6阳性病例8例;应用RT-PCR方法,Her-2阳性表达27例,EGFR呈阳性表达21例,CK5/6阳性病例21例。其中Her-2应用两种方法所得阳性率没有统计学差别;EGFR、CK5/6应用两种方法所得阳性率不同,P<0.01。结论免疫组化方法与RT-PCR方法相比较,乳腺癌组织EGFR、CK5/6等基因在不同组织中的扩增和蛋白过表达具有差异性,Her-2不具有差异性,RT-PCR方法敏感性和准确性更高,但二者具有一致性。Objective To discuss the application in the treatment and prognosis of breast canc- er with immunohistochemistry（IHC） and real-time quantitative PCR（RT-PCR） for breast canc- er susceptibility gene Her-2、EGFR、CK5/6. Methods The Her-2, EGFR, CK5/6 gene ex- pression levels were detected by IHC and RT-PCR in 41 patients with breast cancer. The sensi- tivity and specificity of two kinds of methods were analyzed and compared. Results Forty-one cases of breast cancer were women. Immunohistochemical methods showed 21 cases of Her-2 positive expression, 8 cases of EGFR positive, and 8 cases of CKS/6-positive; But RT-PCR method showed Her-2 positive in 27 cases, EGFR positive in 21 cases, and CK5/6 positive in 21 cases. Her-2 positive rates were not statistically different with both of these methods （P 〉 0. 05） ; EGFR, CK5/6 positive rates of different methods were statistically significant （ P 〈 0. 05）. Conclusion Detection of EGFR and CK5/6 have difference in amplification and pro- tein expression in different tissues, but Her-2 has not with two methods. RT-PCR method hashigher sensitivity and. accuracy, but their results are consistent.

关 键 词：乳腺癌 易感基因 免疫组化 RT-PCR 

分 类 号：R737.9[医药卫生—肿瘤]