NF-κB信号通路在人牙髓细胞分化中作用的研究  

NF-κB signaling pathway regulates the differentiation of human dental pulp cells

作　　者：王志华[1] 吕海鹏[1] 韩冰[1] 张菁[1] 付蕾[1] 张婧[1] 李丹[1] 张芳[1] 何文喜[1] 

机构地区：[1]第四军医大学口腔医学院,陕西西安710032

出　　处：《牙体牙髓牙周病学杂志》2013年第3期149-152,167,共5页Chinese Journal of Conservative Dentistry

基　　金：国家自然科学基金资助项目(81271125;81070831;30872869)

摘　　要：目的:通过NF-κB(nuclear factor-κB,NF-κB)抑制剂(Pyrrolidine dithiocarbamate,PDTC)研究NF-κB信号通路在人牙髓细胞分化中的作用。方法:组织块酶消化法分离培养人牙髓细胞,并用含有20μmol/L PDTC的矿化液对其进行诱导培养2周,RT-PCR检测PDTC刺激对牙髓细胞矿化相关基因碱性磷酸酶(Alkaline phosphatase,ALP)、骨钙素(Osteonectin,OCN)和骨桥素(Osteopontin,OPN)mRNA表达水平的影响;诱导培养4周后茜素红染色观察PDTC刺激下牙髓细胞形成矿化结节情况。结果:矿化诱导培养2周后,RT-PCR检测显示:PDTC刺激组的ALP、OCN和OPN mRNA的表达水平均明显上调,分别与对照组相比,差异均有统计意义(P<0.05)。矿化诱导培养4周后,茜素红染色显示:PDTC刺激组矿化结节明显多于对照组。结论:PDTC可促进牙髓细胞分化,提示NF-κB信号通路可能具有抑制牙髓细胞分化的作用。AIM： To investigate the effect of nuclear factor-κB （ NF -κB） signaling pathway on the differ-entiation of human dental pulp cells （hDPCs）. METHODS： Tissue block enzymolytic method was used to isolate and culture hDPCs, hDPCs of passage 4 were stimulated with inducing culture medium containing 20 μmol/L of pyrolidine dithiocarbamate （PDTC）, an NF-κB inhibitor. Cells cultured with no PDTC served as the controls. 2 weeks after min- eralization induction culture, mRNA expressions of osteonectin （OCN）, alkaline phosphatase （ALP） and osteopontin （OPN） were detected by RT - PCR. 4 weeks after induction mineral nodules were observed by alizarin red S staining. RESULTS： OCN, ALP and OPN mRNA expressions were significantly increased in the PDTC treated cells （ P 〈 0.05 ）. More mineral nodules were observed in PDTC treated cells than in the controls （ P 〈 0.05 ）. CONCLU-SION： NF-κB signaling pathway might play an inhibitory role on the differentiation of human dental pulp cells.

关 键 词：核转录因子ΚB 吡咯烷二硫基甲酸 人牙髓细胞 分化 

分 类 号：R780.2[医药卫生—口腔医学]