合成气发酵制取乙醇微生物的对比  

Comparison of microorganisms fermenting syngas into ethanol

作　　者：宋安东[1,2] 冯新军[1,2] 王风芹[1,2] 谢慧[1,2] 杨大娇[1,2] 

机构地区：[1]河南农业大学生命科学学院,河南郑州450002 [2]农业部农业微生物酶工程重点实验室,河南郑州450002

出　　处：《生物工程学报》2013年第3期342-349,共8页Chinese Journal of Biotechnology

基　　金：河南省省院科技合作专项资金项目(No.102106000018)资助~~

摘　　要：为了验证富集菌群利用合成气产乙醇的能力,以富集培养获得的4种菌群A-fm 4、G-fm 4、LP-fm 4、B-fm 4为研究对象,在10%接种量和25%接种量两种情况下,与合成气发酵菌株梭状芽胞杆菌Clostridiumautoethanogenum进行对比。结果表明:10%接种量时,A-fm 4、G-fm 4、LP-fm 4、B-fm 4与C.autoethanogenum乙醇产量分别为349.15、232.16、104.25、79.90和26.99 mg/L;25%接种量发酵时,乙醇产量分别为485.81、472.73、348.58、272.52和242.15 mg/L。提高接种量能增加乙醇的产量。富集菌群乙醇产量与目前报道的C.autoethanogenum最高产量(259.64 mg/L)相比具有显著优势,提供了一种提高合成气发酵生产乙醇产量的方法。To evaluate the ability of microbial mix-culture fermenting syngas into ethanol, we studied the microbial mix-cultures A-fro 4, G-fro 4, Lp-fm 4 and B-fm 4 obtained by enrichment and compared with Clostridium autoethanogenum DSM10061 with 10% and 25% inoculation size. The results show that, with 10% inoculation size, theethanol production of A-fm 4, G-fro 4, Lp-fm 4, B-frn 4 and C. autoethanogenum were 349.15,232.16, 104.25, 79.90 and 26.99 mg/L respectively. With 25% inoculation size, the ethanol production were 485.81, 472.73, 348.58, 272.52 and 242.15 mg/L respectively. Higher inoculation size will increase the production of ethanol. The tested mix-culture exhibited a significant yield advantage compared with the maximum production of C. autoethanogenum reported in the literature （259.64 mg/L）. This research provided a practical method to improve ethanol production from syngas.

关 键 词：合成气 乙醇 富集 发酵 菌群 

分 类 号：TQ223.122[化学工程—有机化工]