乙酸、糠醛和5-羟甲基糠醛对产酸克雷伯氏菌发酵生产2,3-丁二醇的影响  

Effect of acetic acid,furfural and 5-hydroxymethylfurfural on production of 2,3-butanediol by Klebsiella oxytoca

作　　者：吴晶[1,2] 程可可[2] 李文英[1] 冯杰[1] 张建安[2] 

机构地区：[1]太原理工大学煤科学与技术省部共建国家重点实验室培育基地,山西太原030024 [2]清华大学核能与新能源技术研究院,北京100084

出　　处：《生物工程学报》2013年第3期350-357,共8页Chinese Journal of Biotechnology

基　　金：国家自然科学基金(No.21176141);国家重点基础研究发展计划(973计划)(No.2011CB707406)资助~~

摘　　要：为了解产酸克雷伯氏菌对木质纤维素水解液中主要抑制物的耐受和代谢,考察了产酸克雷伯氏菌发酵生产2,3-丁二醇(2,3-butanediol,2,3-BDO)过程中对3种发酵抑制物乙酸、糠醛和5-羟甲基糠醛(5-hydroxymethylfurfural HMF)的耐受以及抑制物浓度的变化,检测了糠醛和HMF的代谢产物。结果表明:产酸克雷伯氏菌对乙酸、糠醛和HMF的耐受浓度分别为30 g/L、4 g/L和5 g/L。并且部分乙酸可作为生产2,3-丁二醇的底物,在0-30 g/L浓度范围内可提高2,3-丁二醇的产量。发酵过程中产酸克雷伯氏菌可将HMF和糠醛全部转化,其中约70%HMF被转化为2,5-呋喃二甲醇,30%HMF和全部糠醛被菌体代谢。研究表明在木质纤维素水解液生产2,3-丁二醇的脱毒过程中可优先考虑脱除糠醛,一定浓度的乙酸可以不用脱除。To get the tolerability and consumption of Klebsiella oxytoca on major inhibitors in lignocelluloses hydrolysate, we studied the effect of acetic acid, furfural and 5-hydroxymethylfurfural on production of 2,3-butanediol by Klebsiella oxytoca. The metabolites of furfural and 5-hydroxymethylfurfural were measured. The results show that when acetic acid, furfural and 5-hydroxymethylfurfural was individually added, tolerance threshold for Klebsiella oxytoca was 30 g/L, 4 g/L and 5 g/L, respectively. Acetic acid was likely used as substrate to produce 2,3-butanediol. The yield of 2,3-butanediol increased when acetic acid Concentration was lower than 30 g/L. In the fermentation, more than 70% 5-hydroxymethylfurfural was converted to 2,5-furandimethanol. All furfural and the rest of 5-hydroxymethylfurfural were metabolized by Klebsiella oxytoca. It showed that in the detoxification process of 2,3-butanediol production using lignocelluloses hydrolysate, furfural should be given priority to remove and a certain concentration of acetic acid is not need to removal.

关 键 词：产酸克雷伯氏菌 2 3-丁二醇 乙酸 糠醛 5-羟甲基糠醛 抑制物 

分 类 号：TQ923[轻工技术与工程—发酵工程]