涎腺腺样囊性癌中血管生成拟态结构的研究  

A study on vasculogenic-mimicry in salivary adenoid cystic carcinoma

作　　者：付兆臣[1] 关呈超[1] 吴迪[1] 张斌[1] 

机构地区：[1]哈尔滨医科大学附属第二医院口腔外科,150086

出　　处：《实用口腔医学杂志》2013年第2期228-230,共3页Journal of Practical Stomatology

基　　金：黑龙江省教育厅课题资助项目(编号:112521188);国家自然科学基金资助项目(编号:30330580)

摘　　要：目的:探讨涎腺腺样囊性癌(SACC)中是否存在血管生成拟态(VM)。方法:首先将涎腺腺样囊性癌细胞系ACC-M复苏传代,然后按照1×107/ml浓度0.2 ml进行裸鼠臀部皮下注射建立裸鼠模型(n=40),3周后肿瘤长至直径约10 mm,处死裸鼠,切取标本后分别固定保存于4%甲醛溶液和戊二醛中,通过HE染色和CD31染色检测SACC中是否存在VM;并在电镜下观察细胞结构。结果:经HE染色和CD31染色证明组织结构中存在血管生成拟态结构。电镜观察见VM结构中细胞间隙较宽。结论:SACC病理结构中存在血管生成拟态。Objective： To explore vasculogenic mimicry （VM） in salivary adenoid cystic carcinoma （SACC）. Methods： SACC tumor was induced in 40 nude mice by injection of ACC-M cells subcutaneously. 3 weeks after injection, tumor samples were exam- ined by HE staining and CD31 immunohistoehemieal staining for the detection of VM. Cell structure was observed under transmission electron microscope. Results： HE staining and CD31 staining proved VM structure in the SACC tumors. Electron microscopy showed that the space between the cells of VM was wider. Conclusion： VM exists in SACC tumor.

关 键 词：腺样囊性癌 血管生成拟态 CD31免疫组化染色 

分 类 号：R739.8[医药卫生—肿瘤]