蝎毒多肽提取物对人膀胱癌T24细胞增殖抑制作用的研究  

PESV Inhibited Proliferation of Human Bladder Carcinoma T24 Cells

作　　者：侯毅[1,2] 龙俊任[3] 张平[1,2] 龙兵[1,2] 陈晓波[1,2] 董自强[1,2] 

机构地区：[1]三峡大学第一临床医学院 [2]宜昌市中心医院,湖北宜昌443000 [3]四川省阆中市人民医院

出　　处：《天津医药》2013年第3期204-207,共4页Tianjin Medical Journal

基　　金：三峡大学2010年研究生创新基金(项目编号:2010CX059)

摘　　要：目的探讨蝎毒多肽提取物(PESV)对人膀胱癌T24细胞增殖的抑制作用及其机制。方法采用体外培养法培养T24细胞,应用MTT法检测对照组和实验组(不同浓度PESV)处理膀胱癌T24细胞后的增殖变化;倒置显微镜观察其对T24细胞形态的影响;RT-PCR检测实验组(低剂量、中剂量及高剂量)BAX和Bcl-2的mRNA的表达变化。结果:PESV能显著抑制T24细胞的增殖,呈现时间和剂量依赖性,差异具有统计学意义(P<0.01),24h抑制率接近50%的T24细胞生长浓度为75mg/L;低、中及高浓度PESV均可致T24细胞生长明显抑制,出现典型细胞凋亡形态;和对照组比较,实验组BAXmRNA和BAX/Bcl-2mRNA表达升高,Bcl-2mRNA表达下降,差异有统计学意义(P<0.01)。结论 PESV对膀胱癌T24细胞具有明显抑制作用,其诱导T24细胞凋亡的作用可能与上调BAX和下调Bcl-2的表达有关。Objective To investigate the effect of polypeptide extract from scorpion venom （PESV） on the prolifera- tion of human bladder carcinoma T24 cells, and the mechanism thereof. Methods T24 cells were cultured in vitro and treat- ed with different concentration of PEST. The proliferation of cells was detected by MTT assay. The variation of cell morpholo- gy was observed by the invented microscope. RT-PCR was used to detect the variation of BAX mRNA and Bcl-2 mRNA in low dose, middle dose and high dose PEST groups.Results PESV significantly inhibited the proliferation of human bladder carcinoma T24 cells in a dose-dependent and time-dependent manner （P 〈 0.01）. The concentration of inhibited half T24 cells was 75 mg/L. The cell apoptosis was observed in different concentrations of PESV groups. Compared with control group, the expressions of BAX and BAX/Bcl-2 mRNA were gradually increased and the expression of Bcl-2 mRNA was gradually decreased with the concentration of PESV increasing （P 〈 0.01）. Conclusion PESV can inhibit proliferation of human blad- der carcinoma T24 cells and the possible mechanism may be related with the up-regulation of BAX expression and down-reg- ulation of Bcl-2.

关 键 词：膀胱肿瘤 癌 细胞系 肿瘤 细胞凋亡 基因 bcl-2 BCL-2相关X蛋白质 蝎毒多肽提取物 

分 类 号：R730.21[医药卫生—肿瘤]