甲状腺转录因子在宣威肺腺癌中的表达及临床意义  

Expression and Clinical Significance of the Thyroid Transcription Factor 1 in Xuanwei Lung Adenocarcinoma

作　　者：俎云芬[1] 王熙才[2] 刘馨[2] 陈艳[2] 李承文[3] 尚学琴[1] 谢余澄[4] 赵虹园 王继营[1] 

机构地区：[1]云南省第二人民医院肿瘤科,昆明650021 [2]云南省肿瘤医院肿瘤研究所,昆明650118 [3]云南省肿瘤医院科研科,昆明650118 [4]云南省第二人民医院病理科,昆明650021 [5]昆明市盘龙区人民医院急诊科,昆明650021

出　　处：《中国肺癌杂志》2013年第3期131-137,共7页Chinese Journal of Lung Cancer

基　　金：云南省科技厅-昆明医科大学联合专项基金(No.2011FB227;No.2012FB067);国家自然科学基金资助项目(No.81060177)资助~~

摘　　要：背景与目的通过检测宣威肺腺癌细胞株(XWLC-05)中甲状腺转录因子(thyroid transcription factor 1,TTF-1)mRNA和蛋白表达及宣威肺腺癌标本组织TTF-1和Ki-67蛋白的表达,分析宣威肺腺癌中TTF-1与Ki-67表达相关性及对患者预后的影响。方法利用qRT-PCR方法检测XWLC-05中TTF-1 mRNA和免疫组化分析TTF-1及Ki-67蛋白表达。同时收集2008年1月-2012年3月96例手术后宣威肺腺癌组织标本,免疫组化检测TTF-1及Ki-67的蛋白表达。结果 XWLC-05中TTF-1 mRNA低表达,免疫组化TTF-1表达阴性,Ki-67100%表达。96例肺癌患者TNM分期I期-II期66%(63/96)、III期34%(33/96),TTF-1蛋白表达率93%(89/96),Ki-67为70%(67/96),其中高分化腺癌TTF-1阳性表达率98%(31/39),中低分化腺癌89%(51/57)。TTF-1阳性表达随着分化程度的增加而增高(P=0.002),与Ki-67的表达负相关(P=0.01),与性别、年龄、吸烟史及分期无关。Kaplan-Meier生存曲线显示患者中位无进展生存(medianprogression-freesurvival,PFS)与分期、TTF-1及Ki-67表达强度有关,I期-II期PFS高于III期(46个月vs32个月,P=0.001),TTF-1强阳性高于弱阳性和阴性组(45个月vs35个月,P=0.036),Ki-67弱阳性和阴性高于强阳性组(46个月vs40个月,P=0.048)。结论 TTF-1在肺腺癌中可能是一个肿瘤抑制基因,基于它能抑制Ki-67的表达,并且可作为宣威肺腺癌好的预后指标。Background and objective The present study aims to detect the expressions of the thyroid transcription factor 1（TTF-1） mRNA and protein in Xuanwei lung adenocarcinoma line（XWLC-05）.We also investigated the relationship of TTF-1 with Ki-67 protein and analyzed the prognostic value of the TTF-1 protein in Xuanwei lung adenocarcinoma patients.Methods The expression of TTF-1 mRNA was evaluated by quantitative real-time RT-PCR（qRT-PCR）,while proteins of TTF-1 and Ki-67 were detected by immunohistochemistry in XWLC-05.The expressions of TTF-1 and Ki-67 was detected in 96 resected cases of Xuanwei lung adenocarcinoma by immunohistochemistry from January 2008 to March 2012.Results TTF-1 mRNA was low and protein was absent in XWLC-05.The expression of Ki67 protein was 100%（high proliferative activity） in XWLC-05.All samples were Xuanwei lung adenocarcinoma（n=96） and TNM stages were I-II 66%（63/96） and III 34%（33/96）.Immunohistochemical analysis showed the expression of TTF-1 in 89（93%） of 96 and Ki-67 in 69（70%） of 96.The positive rate of TTF-1 protein was 38（96%） of 39 in well-differentiated phenotype and 51（89%） 57 in moderately/poorlydifferentiated phenotype.Patients with strong immunohistochemical expression of TTF-1 were statistically associated with well-differentiated phenotype（P=0.002）,has inverse correlation with Ki-67 expression（P=0.01）,and no correlation with age,sex,smoking history,and stages.The result from the Kaplan-Meier survival analysis showed that stages TTF-1 and Ki-67 were significantly associated with the prognosis of Xuanwei lung adenocarcinoma patients.The median progression-free survival in patients with I-II stages,strong positive expression TTF-1 and negative,and weak expressions Ki-67 groups was remarkably higher than those patients with III stage（46 months vs 32 months,P=0.001）,negative and weak expressions TTF-1（45 months vs 35 months,P=0.036） and strong positive expression Ki-67 groups（46 months vs 40 months,P=0.048）,respectivel

关 键 词：甲状腺转录因子 KI-67 肺肿瘤 预后 

分 类 号：R734.2[医药卫生—肿瘤]