土鳖虫含药血清干预骨髓间充质干细胞的蛋白组学差异  

Effects of eupolyphaga-containing serum on proteomics changes in bone marrow mesenchymal stem cells

作　　者：仲卫红[1] 齐振熙[2] 

机构地区：[1]福建中医药大学附属康复医院,福建省福州市350003 [2]福建中医药大学,福建省福州市350003

出　　处：《中国组织工程研究》2013年第1期98-105,共8页Chinese Journal of Tissue Engineering Research

基　　金：福建省自然科学基金资助(2008J0325)~~

摘　　要：背景:国内外学者对股骨头缺血性坏死做了大量研究,但股骨头缺血性坏死的确切发病机制仍未完全清楚。目的:探讨土鳖虫含药血清干预骨髓间充质干细胞后抑制成脂分化和促进成骨分化的靶位蛋白或相关蛋白。方法:第3代骨髓间充质干细胞分为空白对照组、激素组、土鳖虫组,分别用含空白血清、地塞米松磷酸钠注射液、土鳖虫含药血清+地塞米松磷酸钠注射液的DMEM培养基干预。3组干预6d后运用同位素标记相对和绝对定量技术(iTRAQ)标记3组样本的蛋白,二维液相色谱质谱仪(2DLC-MS/MS)鉴定蛋白并进行相对定量分析。结果与结论:以激素组为对照,在空白对照组中共有15种蛋白质出现显著的表达上调,12种出现下调蛋白;在土鳖虫组中共有16种蛋白质出现显著的表达上调,14种出现显著下调。其中Hspa1L和PrxⅤ蛋白可能是土鳖虫抗细胞凋亡的作用靶点。Serpinh1和ADP/ATP translocase 1蛋白可能是土鳖虫促进成骨分化的作用靶点。BACKGROUND： Avascular necrosis of the femoral head has been much studied. However, the precise pathological mechanism underlying avascular necrosis of the femoral head remains unclear.OBJECTIVE： To investigate the effects of eupolyphaga-containing serum on the target or related proteins inhibiting adipogenic differentiation and promoting osteogenic differentiation of bone marrow mesenchymal stem cells. METHODS： Passage 3 bone marrow mesenchymal stem cells were cultured with DMEM containing blank serum （blank control group）, dexamethasone （hormone group） and eupolyphaga-containing serum （eupolyphaga group）. After 6 days of culture, isobaric tag for relative and absolute quantitation technique was used to quantify proteins from three groups. The proteins were identified and relatively quantitatively analyzed by mass spectrometry. RESULTS AND CONCLUSION： Relative to the hormone group, there were 15 proteins significantly up regulated and 12 proteins significantly down regulated in the blank control group, and there were 16 proteins significantly up regulated and 14 proteins significantly down regulated in the eupolyphaga group. Hspa1L1 and PrxⅤ may be the targets of anti-apoptosis in eupolyphaga. The expression of Serpinh1 and ADP/ATP translocase 1 may be the targets of promoting osteogenic differentiation in eupolyphaga.

关 键 词：干细胞 骨髓干细胞 干细胞与中医药 土鳖虫 分化 含药血清 二维液相色谱质谱仪 差异蛋白质组学 细胞凋亡 省级基金 

分 类 号：R394.2[医药卫生—医学遗传学]