胃癌细胞一树突状细胞融合疫苗抗肿瘤效应的实验研究  

Experimental Study of Anti-tumor Effect of Gastric Carcinoma Celi-Dentric Cell Fusing Vaccine

作　　者：蒋丽[1] 张桂英[2] 

机构地区：[1]湖南省长沙市第四医院消化内科,湖南长沙410006 [2]中南大学湘雅医院消化内科,湖南长沙410008

出　　处：《医学临床研究》2013年第2期313-316,共4页Journal of Clinical Research

摘　　要：【目的】研究胃癌细胞-树突状细胞（Dc）融合疫苗对严重联合免疫缺陷（scID）小鼠胃癌模型的抗肿瘤效应。【方法】聚乙二醇法诱导胃癌细胞BGC-823与成熟DC融合制备疫苗，将SCID小鼠胃癌模型随机均分为4组：BGC-823／DC融合疫苗组、BGC-823＋DC联合组、DC组和PBS组，分别皮下注射相应疫苗、细胞或试剂，检测小鼠脾脏细胞毒性T淋巴细胞（CTL）活性、皮下移植瘤体积、核因子-KB（NF-KB）、基质金属蛋白酶-9（MMP-9）表达和癌细胞侵袭能力即穿膜细胞数。【结果】CTL活性在融合疫苗组、联合组、DC组及PBS组分别为（39．40±3．38）％、（18．07±1．49）％、（14．074±1．32）Vo、（10．70±1.11）％；移植瘤体积在以上四组分别为（165．03±30．96）mm3、（767．65±118．81）mm3、（1025．16±80．90）mnl。、（1314．99士74．03）mm3；NF-KB表达分别为20．0％、70．0％、90．0％、100．0％；MMP-9表达分别为50．0％、80．0％、90．0％、90．0％；穿膜细胞数分别为15．17±4．67，39．73±6．09、59．36±13．87、90．86±4．97。融合疫苗组各检测指标与其他组比较均有显著差异（P〈O．05）。【结论】融合疫苗能有效诱导CTL活化、下调NF—KB、MMP-9表达及癌细胞侵袭能力，产生特异性抗肿瘤免疫。[Objective] To investigate anti-tumor effect of gastric carcinoma cell-dendritic cell （IX]） fusing vac- cine on severe combined immunodeficiency（SCID） in mouse gastric carcinoma model. [Methods] Vaccine was achieved by the fusion of BGC-823 gastric carcinoma cell with maturate DC by using polyethyleneglycol method. SCID mouse gastric carcinoma models were divided randomly and equally into four groups including BGC-823/DC fusing vaccine group, BGC-823＋DC combining group, DC group and PBS group, and then injected subcutaneously with the corre- sponding vaccine, cell or reagent. Cytotoxic T cell（CTL） activity in mouse spleen, volume of subcutaneous transplan- tation tumor, expressions of nuclear factor-~B （NF-~B） and matrix metalloproteinase-9 （MMP-9） and cancer cell inva- sion （i. e. number of countersinking-membrane cells） were detected. [Results] CTL activity in fusing vaccine group, combining group, DC group and PBS group were （39. 404-3. 38）o//00, （18. 074± 1.49）%, （14.07± 1.32）% and （10.70 ±1. 11）%, respectively. Volumes of subcutaneous transplantation tumor in four groups above-mentioned were （165. 034-30.96）mm3, （767.65±118.81）mm3, （1025. 164±80.90）mm3 and （1314. 994±74.03）mm3, re- spectively. Expressions of NF-B were 20.0%, 70.0%, 90.0% and 100.0%, respectively, and those of MMP-9 were 50.0%, 80.0%, 90.0% and 90.0%, respectively. Numbers of countersinking-membrane cells were 15.17：k 4.67, 39.73 ±6.09, 59.36 4±13.87 and 90.86 ± 4.97, respectively. There was Significant difference in the detection indexes between fusing vaccine group and other groups（ P d0.05）. [Conclusion] Fusing vaccine can effectively in- duce the activation of CTL, and down-regulate the expressions of NF-B and MMP-9 and malignant cell invasion, and elicit specific anti-tumor immunity.

关 键 词：胃肿瘤 树突细胞 癌症疫苗 

分 类 号：R735.2[医药卫生—肿瘤]