免疫磁珠法分离并鉴定前列腺癌类干细胞  

Isolation and Identification of Prostate Cancer Stem-Like Cells by Magnetic Active Cell Sorting

作　　者：秦亮[1] 陈安民[1] 郭风劲[1] 宫晨[1] 卞峰[1] 姚斌[1] 廖晖[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院骨科,武汉市430030

出　　处：《中华全科医学》2013年第3期336-337,389,F0003,共4页Chinese Journal of General Practice

基　　金：教育部新教师基金项目(200804871051);湖北省卫生厅青年人才基金(QJX2010-5)

摘　　要：目的利用免疫磁珠法分选并鉴定人前列腺癌类干细胞。方法利用免疫磁珠法从人前列腺癌细胞株PC3中分选CD133+/CD44+细胞,通过免疫组化、流式细胞术、CCK8、平板克隆形成实验及裸鼠成瘤实验鉴定其表面标志物表达情况及肿瘤干细胞特性。结果经免疫磁珠法分选所得CD133+/CD44+细胞比例为0.50%;免疫组化及流式显示其CD44/CD133/整合素α2β1均高表达;其细胞增殖和克隆形成率高于PC3细胞;以低密度注射时,其裸鼠体内成瘤率明显高于PC3细胞。结论利用免疫磁珠法可从人前列腺癌细胞株PC3中高效的分选出具有肿瘤干细胞生物学特性的前列腺癌类干细胞。Objective To isolate and identify the prostate cancer stem-like cells（CSCs） by magnetic active cell sorting（MACS）.Methods CD133＋/CD44＋ cells were selected from PC3 human prostatic cancer cells lines by MACS,and they were identified as CSCs by immunohistochemistry,flow-cytometric analysis,CCK8,colony-forming assay and tumorigenicity assay.Results Sorted cells showed higher proportion of CD133＋/CD44＋ cells,integrinα2β1＋ cells and enhanced tumorigenic potential in vitro and in vivo when seeded at a low density.Conclusion MACS was a simple and effective method to gain prostate CSCs from PC3 cell lines.

关 键 词：肿瘤干细胞 前列腺癌 免疫磁珠分选 

分 类 号：R737.25[医药卫生—肿瘤] R730.21[医药卫生—临床医学]