茶园土壤高活性氨化菌的筛选鉴定及特性研究  

Research on Screening and Identification of Ammonifying Bacterium and Characteristic of Strains from the Soils of Tea Garden

作　　者：韩晓阳[1] 李智[1] 汪强强[1] 张丽霞[1] 

机构地区：[1]山东农业大学园艺科学与工程学院,作物生物学国家重点实验室,山东泰安271018

出　　处：《茶叶科学》2013年第1期91-98,共8页Journal of Tea Science

摘　　要：经过菌株富集培养、分离纯化等一系列步骤,从山东茶园土壤中分离出33株氨化细菌菌株。经过活性比较和菌株分类,从中选出3株不同种属的高活性菌株。通过对其进行形态特征、生理生化指标的测定及16 S rDNA测序,初步鉴定菌株SNT3属于芽孢杆菌属,菌株SNT6属于微小杆菌属,菌株SNT33属于不动杆菌属。同时对菌株的生长环境以及氨化活性进行了初步研究。结果表明:菌株SNT3、SNT6和SNT33适宜的pH依次为5～8.5、6～8.5、5～7;3株菌株的最适宜生长温度为35℃;在氨化能力方面,菌株SNT3高于菌株SNT6、SNT33。By the experimental procedure of enrichment culture and microbial purification, 33 ammonifying bacterial strains were screened from the soil of tea garden in Shandong province. According to the activity comparison and taxonomy of strains, three different species of high activity strains were selected. The strain SNT3 was preliminary identified as Bacillus, SNT6 as Exiguobacterium and SNT33 as Acinetobacter on the basis of the characteristics of strain＇s morphological characteristics, physiological＆ biochemistry and 16 S rDNA sequencing method. Meanwhile, the effects of growth environment and removal ability of organic nitrogen of the strains were measured. The results showed that the optimum pH for SNT3, SNT6, SNT33 is 5.0-8.5, 6.0-8.5, 5.0-7 respectively; the optimum temperature of three strains were 35 ℃, the capacity of organic nitrogen removing of the SNT3 was better than SNT6 and SNT33.

关 键 词：茶园土壤 氨化细菌 鉴定 特性研究 

分 类 号：S571.1[农业科学—茶叶生产加工] S154.3[农业科学—作物学]