大鼠基质金属蛋白酶-1表达质粒对实验性肝纤维化的影响  

Effects of the recombinant plasmid of rat interstitial collagenase on experimental liver fibrosis

作　　者：杨长青[1] 王吉耀[1] 郭津生[1] 刘建军[1] 张驰 

机构地区：[1]上海医科大学中山医院消化内科,200032

出　　处：《中华消化杂志》2000年第5期297-300,共4页Chinese Journal of Digestion

基　　金：国家自然科学基金资助项目!(395 70 336 )

摘　　要：目的　观察重组基质金属蛋白酶 1真核细胞表达质粒对大鼠肝纤维化的影响。方法　运用DNA重组技术 ,构建融合Flag标记多肽的大鼠基质金属蛋白酶 1真核细胞表达质粒 ,经糖化多聚赖氨酸偶联后 ,利用静脉注射将其导入大鼠肝纤维化模型体内 ;通过RT PCR ,Ⅰ、Ⅲ型胶原的免疫组化以及Masson胶原染色观察重组基质金属蛋白酶 1表达质粒对大鼠肝纤维化的影响。结果　重组基质金属蛋白酶 1表达质粒可在大鼠肝脏中表达 ,其表达产物可促进肝脏中Ⅰ、Ⅲ型胶原的降解 (P <0 .0 5 ) ,但对病理形态学的影响尚不显著 (P >0 .0 5 )。结论　重组基质金属蛋白酶 1表达质粒可促进纤维化肝脏中Ⅰ。Objective To observe the effects of recombinant plasmid of rat's interstitial collagenase on experimental liver fibrosis. Methods We constructed the recombinant plasmid of rat's interstitial collagenase fused Flag peptide which can be expressed in eucaryotic cells. After bound to galactose terminal glyco poly L lysine(G PLL), we transferred the recombinant plasmid to the rat's fibrotic liver in vivo by intravenous injection, then observed the effects of the plasmid on the fibrotic liver by RT PCR, immunohistochemistry and the observation of pathology. Results The recombinant plasmid of interstitial collagenase could be expressed in rat liver, the expression of the plasmid could increase the degradation of type Ⅰ and type Ⅲ collagen in the rat's fibrotic liver compared with fibrotic modal group ( P <0.05). However according to the observation of pathology, the recombinant plasmid of interstitial collagenase have little reverse effects on the rat's fibrotic liver compared with fibrotic modal group ( P >0.05). Conclusion The recombinant plasmid of interstitial collagenase have some effects on liver fibrosis, but this effects is limited.

关 键 词：肝纤维化 基质金属蛋白酶-1 实验研究 胶原酶 

分 类 号：R575.2[医药卫生—消化系统]