丹参酮ⅡA对人肝癌细胞HepG2生长的抑制及诱导凋亡的研究  

Study on Tanshinone Ⅱ A restraining and inducing cell death on hepatocelular carcinoma HepG2 cells

作　　者：林立忠[1] 李剑锋 

机构地区：[1]浙江省台州市中心医院肿瘤外科,浙江台州318000 [2]浙江省温岭市人民医院普外科,浙江温岭317500

出　　处：《中国现代医生》2013年第8期4-6,9,共4页China Modern Doctor

基　　金：浙江省医药卫生科技计划(2008A167)

摘　　要：目的研究丹参酮ⅡA对人肝癌细胞HepG2生长的抑制作用及诱导其凋亡的作用,探讨丹参酮ⅡA抑制肿瘤的作用机制。方法将丹参酮ⅡA配制成0.5μg/L、1.0μg/L、2.0μg/L、5.0μg/L、10.0μg/L的浓度,0μg/L为阴性对照。将肝细胞在不同浓度的丹参酮ⅡA中培养24 h、48 h、72 h,采用MTT法检测丹参酮ⅡA对肝癌细胞HepG2的抑制情况,流式细胞仪检测细胞的周期和凋亡情况。结果相同的浓度,随着时间延长,吸光度逐渐下降,而同一时间,随着浓度的增加,吸光度也逐渐下降。相同浓度,随着时间的延长,丹参酮ⅡA对肝癌细胞HepG2生长的抑制率逐渐增加,相同时间,随着浓度的增加,丹参酮ⅡA对肝癌细胞HepG2生长的抑制率也逐渐增加。随着丹参酮ⅡA浓度的增加,G0/G1的比例逐渐升高(2.0μg/mL、5.0μg/mL、10.0μg/mL),与对照组比较差异有统计学意义(P<0.05)。随着丹参酮ⅡA浓度的增加,细胞凋亡率逐渐升高,与对照组比较差异有统计学意义(P<0.05)。结论丹参酮ⅡA对肝癌细胞HepG2的生长具有抑制作用,且具有时间和浓度依赖性,对凋亡的诱导作用具有浓度依赖性。Objective To study effect of Tanshinone Ⅱ A restraining and inducing cell death on hepatocelular carcinoma HepG2 cells. Methods Tanshinone Ⅱ A was made into different concentration （0.5, 1.0, 2.0, 5.0, 10.0 μg/L）, 0μg/L as negative control, hepatocelular carcinoma HepG2 cells were cultured in different concentration of Tanshinone Ⅱ A for 24, 48, 72 h. Restraining of Tanshinone Ⅱ A on hepatocelular carcinoma HepG2 cells were detected by MTT Cell cycle and apoptosis situation were detected by FCM. Results In same concentration, absorhance decreased according to time, and in same time, absorbanee decreased according to concentration increasing. In same concentration, restraining rate increased according to time, and in same time, restraining rate increased according to concentration increasing. According to concentration increasing, ratio of G0/G1 was higher, which in concentration of 2.0 μg/mL, 5.0 μg/mL, 10.0 μg/mL were higer than control group （P 〈 0.05）. Apoptosis rate increased by concentration increasing, which in concentration of 2.0 μg/mL, 5.0 μg/mL, 10.0μg/mL were higer than control group （P 〈 0.05 ）. Conclusion Tanshinone Ⅱ A shows inbibitional effect on hepatocelular carcinoma HepG2 cells, withtime and concentration dependence. The apoptosis induced effect shows concentration dependence.

关 键 词：丹参酮ⅡA 肝癌细胞 HEPG2 凋亡 

分 类 号：R735.7[医药卫生—肿瘤]