三氧化二砷联合木黄酮抑制CML细胞增殖的研究  

Effects of As_2O_3 in combination with genistein on proliferation of the chronic myelocytic leukemic cells and its mechanism

作　　者：张日[1] 支雅军[1] 朱子玲[1] 仇红霞[1] 夏学鸣[1] 

机构地区：[1]苏州医学院附属第一医院,江苏省血液研究所,215006

出　　处：《江苏医药》2000年第10期763-765,共3页Jiangsu Medical Journal

基　　金：苏州医学院附属第一医院青年骨干基金!(HY007)

摘　　要：目的　探索三氧化二砷 (As2 O3)联合酪氨酸激酶抑制剂木黄酮抑制慢性粒细胞性白血病 (CML)细胞增殖的效应及可能机制 ,为临床应用提供理论依据。方法　以CML细胞系K5 6 2为模型 ,通过细胞增殖检测、形态学观察、肿瘤集落形成和琼脂糖凝胶电泳手段 ,观察比较As2 O3 与木黄酮对CML细胞的增殖抑制效应。结果　经≥ 2 5 μmol/LAs2 O3 和 5mg/L木黄酮处理 2天后的K5 6 2细胞可出现增殖受抑和凋亡的形态学改变及DNA片段化 ,二者具有协同抑制作用。结论　As2 O3 与木黄酮能分别或协同抑制K5 6 2细胞生长 。Objective To explore the effects of As 2O 3(arsenic trioxide)in combination with genistein which is an inhibitor of protein tyrosine kinases on proliferation of the chronic myelocytic leukemia(CML) cells and its mechanisms.Methods K562 cell line was used as in vitro model.The anti proliferation effects of As 2O 3 and genistein were studied by the cell proliferation,cell morphology,tumor colony formation,and DNA gel electrophoresis.Results Treatment with As 2O 3 of ≥2 5μmol/L and genistein of 5mg/L for two days could inhibit the cell proliferation and induce the apoptotic morphology and DNA fragmentation of K562 cells.Both of them showed synergic phenomenon.Conclusion As 2O 3 and genistein can inhibit the growth of K562 cells respectively or synergistically.The mechanism of growth inhibition might be associated with induced apoptosis.

关 键 词：慢性粒细胞性白血病 三氧化二砷 木黄酮 治疗 

分 类 号：R733.72[医药卫生—肿瘤]