人乳头瘤病毒16型L1基因重组表达质粒的构建及在E.Coli宿主中的表达与鉴定  

Expression and Identification of L1 Gene of Human Papilloma Virus Type 16 in E.coli

作　　者：林道红[1] 凌虹[1] 庄敏[1] 郭淑元[1] 马培林[1] 谷鸿喜[1] 

机构地区：[1]哈尔滨医科大学微生物学教研室,150086

出　　处：《中国公共卫生》2000年第10期875-876,共2页Chinese Journal of Public Health

基　　金：黑龙江省重大攻关课题

摘　　要：构建 pGEX4T 1 HPV16L1重组表达系统 ,为进一步研制基因工程疫苗奠定基础 ,采用PCR方法扩增HPV16中国分离株L1外源基因片段 ,pGEX4T 1为表达载体 ,构建pEGX4T 1 HPV16L1重组表达系统 ,在大肠杆菌宿主BL(2 1)中 ,经IPTG诱导 ,表达融合蛋白GST L1,经SDS PAGE电泳和Westernblot进行鉴定结果表明 ,成功构建pEGX4T 1 HPV16L1重组表达质粒并获得高效表达并经Westernblot鉴定为L1外源蛋白。提示 :所构建的 pGEX4T 1It has been established that the infection of human papilloma virus type 16(HPV16)has highly colse relation with the occurring of women cervical carcinoma.And it has great practical significance to explore HPV16 genetic vaccine which can prevent the infection of the same type virus.Method PCR method was applied to amplify L1 gene fragment from HPV16L1 chinese isolate.The recombinant pGEX4T 1 HPV16L1 was constructed from L1 gene fragment and the pGEX4T 1 vector.After transducting to host BL21,IPTG inducing,L1 protein was identified by SDS PAGE and Western blot.Results The recombinant pGEX4T 1 HPV16L1 was successfully identified and L1 protein was expressed with high quantity.Conclusion pGEX4T 1 HPV16L1 could express L1 protein with high production which was identified through western blot identification.

关 键 词：人乳头瘤病毒16型 质粒构建 L1基因表达 鉴定 

分 类 号：R373.9[医药卫生—病原生物学]