大豆异黄酮抑制BCap-37乳腺癌细胞增殖的作用研究  

Antiproliferative Effect of Soybean Isoflavone on Human Breast Cancer Cells BCap-37

作　　者：杨镇洲[1] 朱俊东[1] 糜漫天[1] 张乾勇[1] 周东明[1] 郎海滨[1] 

机构地区：[1]第三军医大学营养卫生教研室,重庆400038

出　　处：《中国公共卫生》2000年第10期913-914,共2页Chinese Journal of Public Health

基　　金：国家自然科学基金资助项目

摘　　要：采用二羟异黄酮、三羟异黄酮处理BCap - 37细胞 1～ 4d后 ,以生长曲线、3 H -TdR掺入试验反映其增殖活力 ,用流式细胞仪检测其对细胞周期的影响。结果显示 :1～ 9×10 5mol/L三羟异黄酮处理BCap - 37细胞 3～ 4d后 ,对BCap -37细胞有增殖抑制作用 ,且与剂量及培养时间成正相关 ,而在相同剂量条件下二羟异黄酮对BCap - 37细胞生长无抑制作用。三羟异黄酮处理BCap - 37细胞 4d后 ,BCap - 37细胞的增殖指数明显下降 ,细胞主要阻滞于G1期。提示 :三羟异黄酮对BCap -BCap-37 cells were given 10μmol/L,30μmol/L,90μmol/L of daidzein and genistein 1～4 days.Growth curve test and the incorpoation of 3H-TdR served as the index to reflect prolifertive activity of BCap-37 cells.Cell cycle of BCap-37 cells were measured by Flow Cytometery.It was showed that BCap-37 cells were inhibitied after they were given 1～9×10 5mol/L of genistein 3～4 days.Antiproliferative activity of genistein was positively related to cultivtive time and dose.Whereas daidzein was not effect proliferation of BCap-37 cells at the same dose.Antiproliferative activity of genistin was significant at 3×10 5mol/L.Proliferation index of BCap-37 cells was obviously decreased after they were exposured to genistein for 4 days.BCap-37 cells were blocked in G 1 phase.The results suggest that Genistein can inhibit proliferation of BCap-37 cells.

关 键 词：大豆异黄酮 乳腺癌 细胞增殖 BCAP-37 

分 类 号：R737.9[医药卫生—肿瘤] R73－36[医药卫生—临床医学]