伴胃肠动力障碍的重症急性胰腺炎大鼠结肠黏膜下神经节一氧化氮合酶阳性神经元的变化  

Change of nitric oxide synthase immunoreactive neurons in colonic submucous ganglia of rats with severe acute pancreatitis complicated with gastrointestinal dysmotility

作　　者：袁园[1] 林中[1] 李小玲[1] 

机构地区：[1]桂林医学院附属医院消化内科,桂林541001

出　　处：《郑州大学学报（医学版）》2013年第1期47-50,共4页Journal of Zhengzhou University(Medical Sciences)

基　　金：广西壮族自治区卫生厅中医药科技专项课题GZKZ10-125;广西壮族自治区科学基金资助项目桂科回0342027

摘　　要：目的:探讨伴有胃肠动力障碍的重症急性胰腺炎(SAP)大鼠结肠黏膜下神经节一氧化氮合酶(NOS)阳性神经元的改变。方法:20只SD大鼠随机分为假手术组与模型组,用逆行胰胆管注射牛磺胆酸钠的方法制作大鼠SAP模型。24h后,测定2组小肠推进比、结肠蠕动频率、粪便含水量,并进行胰腺病理评分;制作结肠黏膜下神经丛全层标本,运用免疫荧光技术观察黏膜下神经节NOS阳性神经元的变化。结果:与假手术组相比,模型组小肠推进比减少[(41.04±4.36)%vs(14.54±2.30)%,t=15.477,P<0.001],结肠蠕动频率减慢[(13.40±1.35)min-1vs(4.00±0.93)min-1,t=16.748,P<0.001],粪便含水量降低[(41.56±6.69)%vs(12.98±6.56)%,t=8.869,P<0.001],胰腺病理评分增高[(1.20±0.63)vs(15.75±1.72),t=23.569,P<0.001],但其结肠黏膜下NOS阳性神经元计数无明显改变[(22.49±5.14)%vs(26.36±2.57)%,Z=-1.955,P=0.055]。结论:伴胃肠动力障碍的SAP大鼠结肠黏膜下NOS阳性神经元可能未发生明显重塑。Aim：To investigate the change of nitric oxide synthase（NOS） immunoreactive neurons in colonic submucous ganglia of rats with severe acute pancreatitis（SAP） accompanied by gastrointestinal dysmotility.Methods：Twenty SD rats were randomly divided into two groups：sham operation group and model group.SAP model was established by retrograde injecting sodium taurocholate into the bile-pancreatic duct.After 24 h,small intestinal transit index,frequency of colonic peristalisis,water content of the fecal matter,and pathological damage of pancreas were measured.Whole-mount samples were prepared and double immunofluorescence was employed to observe NOS immunoactive neurons in colonic submucous ganglia.Results：Compared with sham operation group,small intestinal transit index,frequency of colonic peristalisis and water content of fecal matter of the model group were significantly lower[（41.04±4.36）% vs （14.54±2.30）%,（13.40±1.35） min-1 vs （4.00±0.93） min-1,（41.56±6.69）% vs （12.98±6.56）%,t=15.477,16.748,8.869,P0.001],and score of pancreatic pathological damage was higher[（1.20±0.63） vs （15.75±1.72）,t=23.569,P0.001],however,there was no significant changes in NOS immunoreactive neurons count[（22.49±5.14）% vs （26.36±2.57）%,Z=-1.955,P=0.055].Conclusion：NOS immunoreactive neurons in colonic submucous ganglia may be no significant plasticity in SAP rats complicated with gastrointestinal dysmotility.

关 键 词：重症急性胰腺炎 黏膜下神经节 一氧化氮合酶 胃肠动力障碍 大鼠 

分 类 号：R576[医药卫生—消化系统]