内霉素诱发肝性脑病大鼠脑超微结构观察  

OBSERVATION ON CEREBRAL ULTRASTRUCTURE OF HEPATIC ENCEPHALOPATHY INDUCED BY ENDOTOXIN IN CIRRHOTIC RATS

作　　者：李夏青[1] 韩德五[1] 

机构地区：[1]山西医科大学病理生理学教研室,太原030001

出　　处：《解剖学杂志》2000年第5期439-442,共4页Chinese Journal of Anatomy

摘　　要：目的：观察内毒素诱发肝硬化大鼠发生肝性脑病时血脑屏障超微结构变化。方法：用小剂量内毒素（３ ｍｇ／ｋｇＢＷ）一次性腹腔内注射诱发肝硬化大鼠发生肝性脑病。取部分大脑皮层组织制备超薄切片，在电子显微镜下观察其超微结构变化。结果：肝性脑病大鼠脑微血管明显扩张，内皮皱折增多，内皮下基底膜电子密度降低，甚至局部溶解消失。微血管周围星状胶质细胞突起及皮层内胶质细胞明显肿胀，细胞器成分减少，基质解聚。结论：内毒素诱发肝性脑病时，脑超微结构确有明显变化。这些变化提示，肝性脑病发生时，血脑屏障通透性增加；同时，脑胶质细胞呈现水肿。内毒素血症有可能是各种肝性脑病发生机制学说的共同基础。Objective：The cerebral ultrastrunctural changes of hepatic encephalopathy(HE) in cirrhotic rats induced by endotoxin injection were investigated. Methods: Hepatic encephalopathy were precipated by endotoxin injection peritoneally into the cirrhotic rats(3 mg/kg BW). The ultrathin section from the cerebral cortex tissue were prepared and observed under electron microscopy. Results: The mini blood vessels in the cortex of HE rats were expanded. There was an increase of the cytoplasm folds of endothelium. The basement membrane under endothelial cells showed decreased electronic density or local degradation. The astrocytes and their processes around mini blood vessels expressed a varity of edema, there was a decrease of cytoplasmic organells and appearance of matrix degradation. Conclusion: There are obvious changes in the cerebral ultrastruncture in HE induced by endotoxin injection into cirrhotic rats. The changes imply that the there is a increased permeability of blood-brain barrier(BBB) in HE. Meantime, the glial cells show edema. The development of endotoxemia in HE may be the common base of all kinds of hypothesis about the pathogenesis of HE.

关 键 词：内毒素 肝硬化 肝性脑病 超微结构 

分 类 号：R361.3[医药卫生—病理学]