酮替芬对大鼠脑缺血再灌注损伤的保护作用  

Protective effect of ketotifen on cerebral ischemia-reperfusion in rats

作　　者：张朝霞[1] 汤允昭[2] 

机构地区：[1]山西医科大学口腔系,太原030001 [2]山西医科大学药理学教研室

出　　处：《山西医科大学学报》2000年第5期390-391,共2页Journal of Shanxi Medical University

摘　　要：目的　探讨酮替芬对大鼠急性前脑缺血再灌注损伤的保护作用及其机制。方法　采用Pulsinelli四动脉结扎法略加改良制作动物模型。记录EEG ,检测脑水分、脑组织内CK、MDA、SOD、Na+-K+-ATP酶和Ca2 +-ATP酶。结果　酮替芬可以促进模型鼠EEG的恢复 ,减轻脑水肿 ,使脑组织CK释放和MDA含量减少 ,提高SOD、Na+-K+-ATP酶和Ca2 +-ATP酶的活性。结论　酮替芬对脑缺血再灌注损伤有保护作用 ,可能与其减轻脑水肿 ,抗脂质过氧化 ,维持钠钾泵和钙泵功能有关。Objective To investigate the protective effect of ketotifen on acute cerebral ischemia-reperfusion in rats and the mechanism of its action. Methods Pulsinelli four-vessel occlusion method was used to make the cerebral ischemia-reperfusion model of rats. The EEG was recorded.The cerebral water content(BWC),CK and MDA contents of brain tissue,and the activity of SOD,Na +-K +-ATP enzyme and Ca 2+ -ATP enzyme were tested. Results By using ketotifen in model rats the recovery of EEG was promoted,BWC and CK contents were reduced and the activity of SOD, Na +-K +-ATP enzyme and Ca 2+ -ATP enzyme were improved. Conclusion Ketotifen protects the brain tissue from ischemia-reperfusion injury by anti-lipid peroxide reaction, protects the activity of Na +-K +-ATP enzyme and Ca 2+ -ATP enzyme and reduces brain edema and calcium accumulation. [WT5”HZ]

关 键 词：酮替芬 脑缺血 再灌注损伤 腺苷 三磷酸酶 钠 钾 

分 类 号：R743.31[医药卫生—神经病学与精神病学] R364.12[医药卫生—临床医学]