PCR法鉴定Rorcγt^(GFP)突变小鼠子代基因型  

PCR-based genotype identification of filial generation in Rorcγt^(GFP) mutation mice

作　　者：陈嵘祎[1] 阿彩岭[1] 周英[1] 樊翌明[1] 

机构地区：[1]广东医学院附属医院皮肤科,广东湛江524001

出　　处：《广东医学院学报》2012年第6期603-604,610,共3页Journal of Guangdong Medical College

基　　金：国家自然科学基金面上项目(No.81171512);广东医学院博士启动基金(No.B2010014)

摘　　要：目的探讨PCR(聚合酶链式反应)法鉴定RorcγtGFP突变小鼠子代基因型的可行性。方法 Rorcγt(-/-)基因型雄性与Rorcγt(-/+)基因型雌性小鼠产生的6只新生小鼠,取尾部组织提取DNA,进行PCR扩增及琼脂糖凝胶电泳检测PCR产物,根据产物片段大小鉴定新生小鼠基因型。结果 3只小鼠PCR产物具有241bp片段,为Rorcγt(-/-)基因型;其余3只具有174bp及241bp片段,为Rorcγt(-/+)基因型。结论 PCR是检测RorcγtGFP突变品系小鼠基因型的简便。Objective To investigate the feasibility of PCR in the genotype identification of RorcTt^FP mutation mouse offspring. Methods The genome DNA was extracted from the tails of six newborn mice produced by a male RorcTt（-/-） mouse and a female RorcTt（-/＋） mouse. Rorcγt gene fragments were amplified by PCR and then detected by agarose gel electrophoresis. Results Three mice had Roreγt（-/-） genotype with 241 bp gene fi＇agment, while three mice possessed Rorcγt （-/＋） genotype with 174 bp and 241 bp gene fragments. Conclusion PCR-based method is simple and feasible for the genotype identification of RorcγtGFP mutation mouse offspring.

关 键 词：小鼠 核受体 基因突变 聚合酶链式反应 

分 类 号：R394.1[医药卫生—医学遗传学]