用响应面法优化超声波辅助提取紫椴枝皮中的黄酮  

Optimization of ultrasound-assisted extraction of flavonoids in bark of Tilia amurensis Rupr.by response surface method

作　　者：王婧杰[1] 马凤鸣[1] 穆立蔷[1] 

机构地区：[1]东北林业大学林学院,黑龙江哈尔滨150040

出　　处：《经济林研究》2012年第4期113-118,共6页Non-wood Forest Research

基　　金：黑龙江省青年科学基金项目(QC2010013);哈尔滨市科技攻关计划项目(2012AA6BN022)

摘　　要：为给紫椴中功能成分的深入研究提供参考依据,优化超声波辅助提取紫椴枝皮中黄酮类化合物的提取工艺,采用3因素3水平响应面分析方法,研究了超声提取时间、乙醇浓度、料液比对于超声波辅助提取紫椴枝皮中黄酮类化合物产量的影响。结果表明,超声波辅助提取最佳工艺为:超声提取时间26 min,乙醇体积分数43%,料液比(1︰37)g.mL-1。在此工艺条件下总黄酮得率为5.627%。In order to provide references for the researches on the functional components in Tilia amurensis Rupr., and to optimize the ultrasound-assisted extraction process of flavonoids compounds from the bark in T. amurensis Rupr., the influences of ultrasonic time, ethanol concentration and solid-liquid ratio on extraction yield of flavonoids compounds were investigated by response surface method on the basis of single-factor experiments. The results show that the optimum levels of the three significant parameters are ethanol volume fraction 43%, ultrasonic time 26 min and solid- liquid ratio （1 ： 37） g·mL-1. Under the optimum conditions, the extraction yield offlavonoids compounds is up to 5.627%.

关 键 词：超声波辅助提取 黄酮类化合物 紫椴 响应面法 

分 类 号：S687.1[农业科学—观赏园艺]