柠檬酸工业废水微生物多样性研究  

Microbialdiversity in citrate wastewater

作　　者：陈程程[1] 张强[2] 陈贯虹[2] 刘同军[1] 裴振洪 王加宁[2] 祁庆生[4] 

机构地区：[1]山东轻工业学院食品与生物工程学院,山东济南250353 [2]山东省科学院生物研究所,山东济南250014 [3]日照鲁信金禾生化有限公司,山东日照276800 [4]山东大学生命科学学院,山东济南250100

出　　处：《山东轻工业学院学报（自然科学版）》2012年第1期18-22,共5页Journal of Shandong Polytechnic University

摘　　要：用构建16S rDNA的方法对柠檬酸工业收集废水的汇水池以及预酸化调节过程中的微生物菌群进行了研究。结果表明,在汇水池的16S rDNA文库的71个克隆中共有15个OTU,其中与Lactobacillus mucosae最为相近的OTU有8个,共48个克隆,占所有克隆的67%;与Lactobacillus amylovorus相近的OTU有3个,共15个克隆,占所有克隆的21%;与Lactobacillus fermentum相近的OTU有2个,共2个克隆,占所有克隆的2.8%;与Lactobacilluscoleohominis相近的OTU有1个,共3个克隆,占所有克隆的4.2%。预酸化池的95个克隆分为3个OTU,其中与Lactobacillus mucosae相近的有2个OTU,共82个克隆,占所有克隆的86%;Lactobacillus amylovorus有1个OTU,13个克隆,占14%。研究表明汇水池和预酸化池中的微生物有很大的相似性。Bacterial diversity was studied by constructing bacterium 16S rDNA clone libraries from citric acid wastewater mixing pool and pre-acidification pool. The results showed that clones were placed into operational taxonomic unit（ OTU）groups with I〉99% 16S rDNA sequence similarity（ 15 OTUs,71 clones）. There were Lactobacillus mucosae （ 80TUs, 48clones ）, the number of clones accounted for 67%. Lactobacillus amylovorus （30TUs, 15 clones）, the number of clones accounted for 21%. Lactobacillus fermentum（20TUs,2clones）the number of clones accounted for 2.8%. Lactobacillus coleohominis（ 10TU,3 clones ,the number of clones accounted for 4.2%. in citric acid wastewater mixing pool. Lactobacillus mueosae （20TUs,82 clones） the number of clones aceotmted for 86% and Lactobacillus amylovorus （ 10TU, 13 clones） were found in Pre-acidifieation pool, the number of clones accounted for 4. 2%. The result shows that the bacterial in citric acid wastewater mixing pool and Pre-acidifieation pool was much similarly.

关 键 词：柠檬酸废水 16S RDNA 微生物多样性 汇水池 预酸化池 

分 类 号：X703[环境科学与工程—环境工程]