PRL-3调节PI3K信号通路在促进结肠癌细胞增殖侵袭中的作用  

PRL-3 Promotes Proliferation and Invasion of Colon Cancer Cells by Modulating PI3K Signal Pathway

作　　者：张建龙[1] 张萦斐[1] 张育超[1] 毛凯[1] 陈双[1] 

机构地区：[1]中山大学孙逸仙纪念医院普外科,广东广州510120

出　　处：《中山大学学报（医学科学版）》2013年第1期16-21,共6页Journal of Sun Yat-Sen University:Medical Sciences

基　　金：国家自然科学基金(81071761);广东省自然科学基金(10151008901000044);广东省医学科研基金(A2011171)

摘　　要：【目的】研究肝再生磷酸酶-3(PRL-3)的过表达与PI3K信号通路的调节及其与结肠癌细胞增殖侵袭的关系。【方法】构建稳定转染PRL-3基因和空白对照质粒的结肠癌细胞株LoVo-PRL-3和LoVo-VC,用qRT-PCR和Western blot的方法对miR-21及其靶蛋白PTEN的表达进行检测,在稳转细胞株中转染miR-21或对其进行敲除,用CCK-8、Tanswell实验对细胞的增殖侵袭能力的变化进行研究。【结果】LoVo-PRL-3细胞的增殖侵袭能力要强于对照组LoVo-VC细胞。在结肠癌细胞株LoVo-PRL-3中miR-21的表达上调而PTEN的表达被下调,在LoVo-VC细胞中过表达miR-21促进了细胞的增殖侵袭并降低PTEN的表达,而在LoVo-PRL-3细胞中敲除miR-21抑制了细胞的增殖侵袭并能部分恢复PTEN的表达。【结论】PRL-3通过上调miR-21抑制PTEN的表达,调节了PI3K信号通路,从而促进了结肠癌细胞的增殖侵袭。[Objective] To explore the effect of PRL-3 on proliferation and invasion of colon cancer cells through PI3K signal pathway. [ Methods] We stably transfected PRL-3 expressing plasmid and empty plasmid into LoVo colon cancer cells and established two cell lines ： LoVo-PRL-3 and LoVo-VC. We used qRT-PCR and Western blot to detect the expression of miR-21 and PTEN protein in these colon cancer cell lines. Transient transfection of miR-21mimic into LoVo-VC ceils or transient transfection of miR-21 inhibitor into LoVo- PRL-3 cells were performed to evaluate the proliferation and invasive ability of these cells by CCK8 proliferating assay and transwell chamber assay. [ Results ] PRL-3 promoted proliferation and invasion of LoVo- PRL-3 cells compared with LoVo-VC cells. In LoVo- PRL-3 cells, miR-21 was up-regulated and PTEN was down-regulated. Over-expression of miR-21 promoted proliferation and invasion of LoVo-VC cells and down-regulated PTEN, while knocking down of miR-21 or over-expression of PTEN inhibited proliferation and invasion of LoVo-PRL-3 cells and restore the expression of PTEN. [ Conclusions] Down-regulation of PTEN through up-regulating of miR-21 by PRL-3, which cumulating in enhancing signal of PI3K pathway, promoted proliferation and invasion of colon cancer cells.

关 键 词：肝再生磷酸酶-3 MIR-21 PTEN 

分 类 号：R735.35[医药卫生—肿瘤] R73-37[医药卫生—临床医学]