前列腺素E_2体外可促进人CD34^+细胞增殖  

Prostaglandin E_2 Promotes Human CD34^+ Cells Proliferation

作　　者：赖淑萍[1] 戚永磊[1] 许多荣[1] 

机构地区：[1]中山大学附属第一医院血液内科,广东广州510080

出　　处：《中山大学学报（医学科学版）》2013年第1期22-27,共6页Journal of Sun Yat-Sen University:Medical Sciences

基　　金：国家自然科学基金(30670997);广东省科技计划项目(2011B031800120)

摘　　要：【目的】探讨前列腺素E2(PGE2)体外对人CD34+细胞增殖的影响及其可能机制。【方法】用mini-MACS免疫磁珠分选系统分选人CD34+细胞,并应用流式细胞术鉴定其纯度;将5×103/孔的CD34+细胞用不同浓度的dmPGE2及阴性对照无水乙醇干预后,用集落形成实验检测红系爆式集落形成单位(BFU-E)和粒-单核系集落形成单位(CFU-GM)形成情况;将1×106/mL的CD34+细胞用不同浓度的dmPGE2及无水乙醇干预后,分别用流式细胞术检测细胞周期分布、real time-PCR检测survivin-mRNA水平、Western blot检测胞浆内β-catenin和survivin蛋白表达情况。【结果】人CD34+细胞阳性分选后经流式细胞术鉴定其纯度达95%以上,符合实验要求;1μmol/L dmPGE2干预后,人CD34+细胞形成的BFU-E及CFU-GM数目较其他组明显增多,差异具有统计学意义(P<0.05);1μmol/L dmPGE2干预后,人CD34+细胞进入细胞周期S/G2M期的比例平均是对照组的3.7倍,差异具有统计学意义(P<0.001);1μmol/L dmPGE2干预组人CD34+细胞内survivin-mRNA和survivin蛋白以及β-catenin蛋白表达较其他组明显增多。【结论】PGE2在体外可促进人CD34+细胞增殖,其机制可能与PGE2促进CD34+细胞内survivin、β-catenin表达从而促使部分CD34+细胞由静止期进入分裂期有关。[ Objective ] To investigate the effects and the underlying mechanism of prostaglandin E2 （PGE2） on the proliferation of human CD34＋ cells. [ Methods ] Human CD34＋ cells were isolated by MACS microbead kits, and the purity was identified by flow cytometry. Then 5 ＋103/well human CD3g＋ cells were treated with different concentration dmPGE2. Two hours after cells exposure to ice, erythroid burst forming unit （BFU-E） number and granulocyte monocyte colony forming unit （CFU-GM） number were evaluated by colony-forming assay. Furthermore, cell cycle distribution was analyzed by flow cytometry, and the expression of survivin mRNA, ＋-catenin and survivin protein of human CD34＋ cells was detected by QRT-PCR and Western blot, respectively. [Results] The percentage of CD3g＋ cells in the whole isolated cells was up to 95%. The number of BFU-E and CFU-GM in the 1 ＋mol/L dmPGE2- treated group was higher than that in the control group and that in the other treatment group （P 〈 0.05）. The proportion of human CD34＋ ceils which entered into S/G2M phase was 2.18 times of the control group （P 〈 0.05）. The expression level of survivin mRNA, survivin protein andlS-catenin protein was also elevated significantly after exposure to 1 μmol/L dmPGE2. [ Conclusion] PGE2 promotes human CD34＋ cells proliferation. The underlying mechanism was involved in more quiescent CD34＋ cells entering into cell division cycle, which was mediated by the elevated survivin and β-catenin after exposure to dmPGE2.

关 键 词：异基因造血干细胞移植 植入失败 PGE2 造血干细胞 增殖 

分 类 号：R551.3[医药卫生—血液循环系统疾病]