脊髓水通道蛋白1在大鼠神经病理性疼痛中的作用  

The role of aquaporin 1 in spinal cord of rat with neuropathic pain

作　　者：邢纪斌[1] 莫雪莹[2] 程智刚[2] 彭志勇[3] 王云姣[2] 黑子清[1] 谢咏秋[2] 郭曲练[2] 鄢建勤[2] 蔡宏伟[2] 

机构地区：[1]中山大学附属第三医院麻醉科,广东广州510630 [2]中南大学湘雅医院麻醉与重症医学教研室,湖南长沙410008 [3]南方医科大学附属南海医院麻醉科,广东佛山528200

出　　处：《中国现代医学杂志》2013年第1期1-7,共7页China Journal of Modern Medicine

基　　金：国家自然科学基金(No:30872427)

摘　　要：目的探讨脊髓水通道蛋白1（aquaporin1，AQP1）在大鼠坐骨神经慢性压迫性损伤（CCI）所致神经病理性疼痛中的作用。方法雄性SD大鼠25只，随机分为5组，每组5只：A组：大鼠建立CCI模型后观察4天；B组：大鼠建立CCI模型后观察7天；C组：大鼠建立CCI模型后观察14天；D组：正常对照组，正常饲养观察16天（术前2天＋术后观察14天）；E组：假手术组：暴露左侧坐骨神经中段仅绕线，不结扎，术后观察14天。各组大鼠在建模前1天、建模后第1、4,7、14天测量所有未处死大鼠双后肢的热痛阈（PWTL）和机械痛阈（PwMT）。达到观察时间取大鼠L。节段脊髓，免疫组化法观察AQPl在脊髓的定位分布和表达变化，RealtimePCR法检测脊髓AOP1的mRNA表达变化。结果与同组右侧脊髓背角比较，和c组大鼠左侧脊髓背角AQp1阳性颗粒的平均光密度和面积增高（P〈0．05）。A、B、c组大鼠的脊髓背角AQP1阳性颗粒的面积高于正常对照组和假手术组（P〈0．05），D组与E组之间无差别（P〉0．05）。与正常组和假手术组比较，A、B和C组大鼠脊髓AQPlmRNA含量显著增高（P〈0．05），D组与E组之间无差异（P〉0．05），A组、B组、C组大鼠脊髓AQPl的mRNA含量分别为D组含量的1．688、4．876和5．806倍。结论大鼠脊髓AQP1可能参与神经病理性疼痛的形成和发展。[Objective] To investigate the role of aquaporin 1 in neuropathic pain induced by sciatic nerve chronic constriction injury （CCI） in rat. [ Methods ] Twenty-five healthy male SD rats were randomly divided into five groups, each of five. Mechanical and thermal pain threshold were measured during the first day, the third day, the sixth day, the ninth day, the sixteenth days, respectively, when the study began. CCI model was established in fifteen rats （groups A, B, C）. Five rats were selected as control group （group D）. Five rats were selected as sham-op- eration group （group E）. Lumbar spinal cords in all groups were dissected out on the sixth day （group A）, the ninth （group B） and the sixteenth （groups C, D, E）. The localization of AQP1 was demonstrated by immunohistoehemieal assay and the levels of mRNA expression of AQPI in L4~s segmental spinal cord were detected by relative real-time fluorescent quantitative PCR assay. [Results] AQP1 positive particles were presented in neuronal cytoplasm widespreadly, especially concentrated in the left spinal cord dorsal in lumbar segment of spinal cords in rats in each group. The expression level of AQP1 in CCI groups was more intensive than the others. The mean AQP1 mRNA con- tent in CCI groups was higher than that in control group and sham group （P 〈0.05）. The expression content of AQP1 mRNA in Group A, B, C were 1.688, 4.876, 5.806 times than that in control group respectively. [Conclusion] The mRNA and the protein expression of AQP1 were increased in CCI rats. AQP1 signal pathway may play a role in the pathogenesis of neuropathic pain in rats induced by chronic constriction injury to the sciatic nerve.

关 键 词：水通道蛋白1 神经病理性疼痛 脊髓 

分 类 号：R-332[医药卫生]