艾灸对脾虚大鼠空肠组织ATP含量和ATP酶活性的影响  

The effect of moxibustion on content of ATP and activities of ATPase in jejunal tissues of spleen-deficiency rats

作　　者：彭艳[1] 易受乡[1] 林亚平[1] 彭芬[1] 龙奕文[1] 常小荣[1] 徐斌[2] 

机构地区：[1]湖南中医药大学针灸推拿学院经穴与脏腑相关重点研究室(国家中医药管理局三级实验室),湖南长沙410007 [2]南京中医药大学中医学一级学科,江苏南京210046

出　　处：《中国现代医学杂志》2013年第1期8-13,共6页China Journal of Modern Medicine

基　　金：江苏高校优势学科建设工程项目南京中医药大学中医学一级学科开放课题(No:YS2012ZYX414);湖南省教育厅(No:11C0965)

摘　　要：目的观察艾灸对脾虚大鼠血清D木糖含量、空肠组织ATP含量及ATP酶活性的影响,探讨艾灸对ATP生成与膜蛋白含量的影响,分析艾灸温补脾胃改善脾虚证的作用机理。方法 40只SD大鼠随机分为4组:A空白对照组,B脾虚模型组,C艾灸穴位组(脾虚+艾条温和灸组),D中药对照组(脾虚+四君子汤组),每组10只。200%大黄水浸剂灌胃造成脾虚大鼠模型,艾灸足三里、中脘、关元、脾俞、胃俞等穴,间苯三酚法测定血清D-木糖含量,比色法测定空肠组织三磷酸腺苷(ATP)含量及Na+-K+-ATP酶(Na+-K+-ATPase)和Ca2+-Mg2+-ATP酶(Ca2+-Mg2+-ATPase)活性。结果与A组比较,B组脾虚模型大鼠血清D-木糖含量、空肠组织ATP含量、Na+-K+-ATPase和Ca2+-Mg2+-ATPase活性均显著降低(P<0.05或P<0.01);与B组比较,经艾灸足三里等穴或四君子药液灌胃治疗的C、D两组血清D-木糖含量、空肠组织ATP含量、Na+-K+-ATPase和Ca2+-Mg2+-ATPase活性均显著增高(P<0.05或P<0.01)。D组略优于C组,两组比较差异无显著性。结论艾灸能显著增加脾虚大鼠血清D-木糖含量,增加脾虚大鼠小肠组织ATP含量、ATP酶活性,提示艾灸可促进脾虚模型大鼠小肠上皮细胞ATP的生成,增加细胞膜蛋白的含量,改善物质跨膜转运,从而改善小肠吸收功能,促进脾虚症状恢复。[ Objective ] To observe the effect of moxibustion on content of D-xylose in serum, content of ATP and activities of ATPase in jejunal tissues of spleen-deficiency rats, and explore the effect of moxibustion on pro- duction of ATP and content of membrane proteins, to analyze the mechanism of moxibustion reinforcing spleen- stomach. [ Methods ] Forty healthy Sprague Dawley rats were randomly assigned into four groups, namely group A （blank group）, B （model group）, C （moxibustion group） and D （herbs group）. The animal model of spleen-deficiency was established by intragastric administration with Chinese Rhubarb infusion. The rats in group A and B, and D served as the restraint, model, and intragastric administration with sijunzi decoction, respectively, while those in group C received moxibustion at zusanli, zhongwan, guanyuan, pishu and weishu points. The content of D-xylose in serum was detected by phloroglucinol method. Colorimetry method was used to detected content of ATP and activi- ties of Na＋-K＋-ATPase and Ca2＋-Mg2＋-ATPase in jejunum tissues. [ Results ] Compared with group A, the content of D-xylose in serum, content of ATP, activities of Na＋-K＋-ATPase and Ca2＋-Mg2＋-ATP in jejunum tissues were de- creased significantly （P 〈0.05 or P 〈0.01）. Compared with group B, the content of D-xylose in serum, content of ATP, activities of Na＋-K＋-ATPase and Ca2＋-Mg2＋-ATP in jejunum tissues were increased significantly （P 〈0.05 or P 〈0.01）. There was no significant difference between group C and D. [ Conclusions] Moxibustion could increase the content of D-xylose in serum and ATP in jejunum tissues, as well as activities of Na＋-K＋-ATPase and Ca2＋-Mg2＋- ATP of spleen-deficiency, which suggested that moxibustion could enhance absorption functions of small intestine to relieve spleen-deficiency symptoms by promoting the production of ATP in intestinal epithelial cells, increasing the content of membrane proteins, and improving transmembrane transport.

关 键 词：艾灸 脾虚 ATP ATP酶 四君子汤 

分 类 号：R-332[医药卫生]