曲古抑菌素A对人乳腺癌MCF-7细胞抑制作用的实验研究  

Study of TSA inhibiting proliferation in breast carcinoma cell line MCF-7

作　　者：张秀梅[1] 刘超[1] 刘阳[1] 肖建英[1] 

机构地区：[1]辽宁医学院生物化学教研室,辽宁锦州121000

出　　处：《中国现代医学杂志》2013年第2期50-53,共4页China Journal of Modern Medicine

基　　金：辽宁省教育厅重点实验室项目(No:LS2010101)

摘　　要：目的探讨曲古抑菌素A(TSA)对乳腺癌细胞系MCF-7细胞生长增殖、细胞周期相关基因CDK4、Cyclin D1、Rb表达的影响。方法分别以不同浓度的TSA处理MCF-7细胞,采用流式细胞仪检测细胞周期;用RT-PCR方法检测CDK4、Cyclin D1、Rb mRNA的表达水平;用Western blotting法检测MCF-7细胞的CDK4、Cyclin D1、Rb蛋白表达。结果各组细胞均随着药物浓度的升高,S期细胞在细胞周期中所占比例逐渐减少,G1期细胞则明显增加(P<0.01),细胞滞留在G1期;经不同浓度TSA处理的细胞CDK4和Rb mRNA表达水平没有明显变化;Cyclin D1的mRNA表达水平显著下降。CDK4蛋白表达水平也没有明显变化,Cy-clin D1和pRb磷酸化蛋白的水平明显下降。结论 TSA抑制乳腺癌MCF-7细胞的增殖可能是通过下调Cyclin D1和Rb蛋白的磷酸化水平来实现的。[Objective] To study the effects of trichostatin A（TSA） on cell proliferation and expressions of CDK4, Cyclin D1 and Rb in MCF-7 cells. [Methods] MCF-7 cells were treated with different dose of TSA, cell cycle was measured by flow cytometry; the expression levels of CDK4, Cyclin DI and Rb mRNA were determined by RT-PCR; expression of CDK4, Cyclin DI and pRb protein were determined by Western blot-ting. [Results] After cells were treated with TSA, Flow cytometry analysis showed the percent of G1 phase cells was significantly higher than that of the control groups and the percent of S phase cells was lower than that of the control groups. The cell cycle was arrested at GI stage; the mRNA expression of the CDK4 and Rb were no distinct drange; the mRNA expression of the Cyclin D1 was decreased; no distinct change in the expressions of CDK4 protein was observed; level of Cyclin D1 and pRb protein phosphorylation were de-creased dramatically. [Conclusion] TSA may inhibite the proliferation of MCF-7 cells by down-regulating Cyclin DI and pRb protein the phosphorylation

关 键 词：曲古抑菌素A MCF-7 细胞周期 CDK4 Cyclin D1 PRB 

分 类 号：R736.1[医药卫生—肿瘤] Q253[医药卫生—临床医学]