基质金属蛋白酶促进瘢痕疙瘩成纤维细胞迁移及其意义  

Matrix metalloproteinase promote fibroblast cell migration in keloids and significance

作　　者：张鹏[1] 纪亮[1] 张翠香[1] 侯金才[1] 李云峰[2] 李静平[1] 

机构地区：[1]齐齐哈尔医学院解剖教研室,黑龙江齐齐哈尔161006 [2]齐齐哈尔医学院第三附属医院整形美容科,黑龙江齐齐哈尔161006

出　　处：《中国现代医学杂志》2013年第3期11-14,共4页China Journal of Modern Medicine

基　　金：黑龙江省自然科学基金面上项目(No:D200957);黑龙江省齐齐哈尔市科技局社会发展项目(No:SHFZ-9011)

摘　　要：目的通过研究瘢痕疙瘩中基质金属蛋白酶(MMPs)与成纤维细胞迁移的关系,探索从抑制成纤维细胞迁移角度治疗瘢痕疙瘩的新方案。方法切取21例已确诊的瘢痕疙瘩组织为实验组,同例标本附带的正常皮肤组织为对照组,采用酶联免疫吸附实验(ELISA)方法,定量检测成纤维细胞上清中I型前胶原肽(PINP),基质金属蛋白酶-1、-2(MMP-1,MMP-2)及基质金属蛋白酶抑制剂-1(TIMP-1)的含量;向实验组或对照组上清中分别添加MMPs抑制剂(GM 6001),利用Transwell细胞小室实验对成纤维细胞的迁移情况进行评估。结果与正常皮肤成纤维细胞相比,瘢痕疙瘩组织MMP-1、MMP-2、TIMP-1和PINP表达均较高,有显著差异(P<0.01);瘢痕疙瘩成纤维细胞迁徙活动与正常皮肤成纤维细胞相比,有显著差异(P<0.01),而这些成纤维细胞的迁徙活动可被广谱MMPs抑制剂(GM 6001)所抑制,有显著差异(P<0.01)。结论瘢痕疙瘩成纤维细胞高表达基质金属蛋白酶,产生的的基质金属蛋白酶增加了瘢痕疙瘩成纤维细胞的迁徙活动。[Objective] To explore new therapeutic strategy to treat early keloidosis, we examined the role of matrix metalloproteinases （MMPs） in fibroblast cell migration in keloidosis. [Methods] 21 validated keloidosis tis- sues and corresponding normal skin tissues were collected. The protein levels of type I procollagen peptide （PINP）, MMP-1, MMP2, and MMP inhibitor-1 （TIMP-1） in the cultured medium of the fibroblast cells were measured by ELISA. MMPs inhibitor （GM 6001） were added to the supernatant of the experimental group or control group, in vitro cell migration assay was performed using Transwell chamber system. [ Results ] The expression of MMP-1, MMP-2, TIMP-1 and PINP is significantly higher in keloidosis fibroblasts than that in normal tissue fibroblasts （P 〈0.01）; the migratory rate of keloid fibroblasts is also significantly higher than that of normal fibroblasts （P 〈0.01）. Mover over, the motility of these fibroblasts is significantly repressed by MMPs inhibitor GM6001 （P 〈0.01）. [Conclusion] MMPs were significantly highly expressed in keloid fibroblasts and promote fibroblast cell migration in keloids.

关 键 词：瘢痕疙瘩 基质金属蛋白酶 成纤维细胞 细胞迁移 

分 类 号：R62[医药卫生—整形外科]