能与大肠杆菌O157(H7)抗血清交叉凝集的5种细菌的分离和鉴定  

Isolation and Identification of 5 Species Bacteria Cross reacting with E. coli O157 (H7) Anti serums

作　　者：郭维植[1] 程法稷 林成水[1] 黄晓蓉 林杰[1] 董新平[1] 徐海滨[1] 陈亢川[1] 

机构地区：[1]福建省卫生防疫站,福州350001 [2]莆田市城厢区卫生防疫站,福建351100 [3]福州卫生检疫局食检所,福建350001

出　　处：《海峡预防医学杂志》2000年第4期4-6,共3页Strait Journal of Preventive Medicine

基　　金：福建省卫生厅科研基金资助课题! (编号 :960 62 )

摘　　要：[目的 ]了解人兽及食品中能与大肠杆菌 O15 7发生交叉凝集的细菌 ,为防止误诊提供参考。 [方法 ]开展调查 ,用大肠杆菌 O15 7及 H7抗血清、O15 7单克隆抗体进行玻片凝集、生化试验等检测。 [结果 ]从肉类及猪粪中检出 5种 (8株 )肠道杆菌能与 O15 7抗血清发生交叉反应 ,其中 4种为国内外首次报道 ,2株检出菌且能与 H7抗血清凝集 ,4株能产生 L T肠毒素。 [结论 ]畜禽中某些种属的肠道细菌可携带与大肠杆菌 O15 7及 O15 7∶H7相同或相关抗原 ,故在鉴定 O15 7∶H7大肠杆菌时除依血清学外 。Objective] To investigate the bacteria which can cross agglutinate with E. coli O157 from men and animals in order to provide the reference information for reducing misdiagnosis. [Methods] O157:H7 bacteria were isolated in Fuzhou and Putian city, Fujian. The strains isolated were assayed with E. coli O157 and H7 antiserum and O157 monoclonal antibodies (McAb) by glass agglutination and the other biochemical test. [Results] 5 species (8 strains) bacteria were isolated from meat and pig's feces and can cross react with O157 antiserum. The 4 species bacteria of them were firstly report in the world. 2 strains of them can agglutinate with H7 antiserums; 4 strains can produce enterotoxin. [Conclusions] Some species of intestinal bacteria from animal and poultry can carry the same or related antigen with E. coli O157 and O157∶H7. When identifying E. coli O157:H7, serological reaction and biochemical tests even the detection of pathogenic factors are needed simultaneously.

关 键 词：O157大肠杆菌 血清凝集 交叉反应 检测 

分 类 号：R378.2[医药卫生—病原生物学]