芹菜过敏原蛋白Api g 1基因的克隆与表达分析  

Cloning and expression analysis of allergenic protein gene Api g 1 in celery

作　　者：李梦瑶[1] 王枫[1] 侯喜林[1] 蒋倩[1] 王镇[1] 马静[1] 熊爱生[1] 

机构地区：[1]南京农业大学园艺学院/作物遗传与种质创新国家重点实验室/农业部华东地区园艺作物生物学与种质创新重点实验室,江苏南京210095

出　　处：《南京农业大学学报》2013年第2期13-19,共7页Journal of Nanjing Agricultural University

基　　金：国家自然科学基金项目(31272175);教育部新世纪优秀人才支持计划项目(NCET-11-0670);江苏高校优势学科建设项目(2011PAPD);江苏省双创计划项目(2011JSSC)

摘　　要：以2个芹菜(Apium graveolens)品种‘津南实芹’和‘美国西芹’为试验材料,分别克隆出过敏原蛋白Api g 1基因。序列分析表明,‘津南实芹’和‘美国西芹’中的过敏原蛋白基因Api g 1均含有1个480 bp的开放阅读框及1个148 bp的内含子,分别编码159个氨基酸,二者有2个核苷酸位点的差异,编码的氨基酸有1个位点差异。‘津南实芹’和‘美国西芹’的过敏原蛋白Api g 1与胡萝卜、欧芹等植物的过敏原蛋白相似度较高,在保守区域有7个甘氨酸残基,空间结构上由3个螺旋和7个折叠组成。实时定量PCR表达分析显示,该基因在主根中表达较高,茎其次,其他部位表达较弱,具有明显的组织特异性。Bioinformatics approach was utilized for analyzing nucleotide and amino acid sequences of Api g 1, which were cloned from celery（Apium graveolens）eultivars＇ Jinnanshiqin＇ and＇ Meiguoxiqin＇ , respectively. Sequence analysis indicated that the Api g 1 gene contained a 480 bp ORF and a 148 bp intron. There were two nucleotide sites and an amino acid residue differences between two eul- tivars. This allergenic protein had a high homology with other higher plants, such as Daucus carota and Petroselinum crispum. There were 7 Gly amino acid residues in the conservative position. The conserved domain of proteins contained three helixes and seven sheets. Quantitative real-time PCR analysis showed that the gene was tissue-specific expressed mainly in the root and stem in celery.

关 键 词：芹菜 过敏原蛋白 API g 1基因 克隆 实时定量PCR 基因表达 

分 类 号：S636.3[农业科学—蔬菜学]