甘蔗宿根矮化病菌多克隆抗体的制备  

Preparation of antiserum against the bacteria of sugarcane ratoon stunting disease

作　　者：谢晓娜[1,2] 陈明辉[1,2] 周丹[1,2] 杨丽涛[1,2,3] 孙富[1,2] 李杨瑞[2,3] 陈保善[1,2] 

机构地区：[1]广西大学农学院,南宁530005 [2]亚热带农业生物资源保护与利用国家重点实验室,南宁530005 [3]中国农业科学院甘蔗研究中心/广西农业科学院甘蔗研究所/农业部广西甘蔗生物技术(与遗传改良重点实验室/广西甘蔗遗传改良重点实验室/广西作物遗传改良生物技术重点开放实验室,南宁530007

出　　处：《植物病理学报》2013年第2期187-191,共5页Acta Phytopathologica Sinica

基　　金：国家科技支撑计划项目(2007BAD30B00);国家863计划课题(2013AA102604);国际合作项目(2009DFA30820;2013DFA31600);广西自然科学基金创新团队项目(2011GXNSFF018002);广西自然科学基金重点项目(2012GXNSFDA053011);自治区主席科技资金项目(11166-02);广西科学研究与技术开发计划项目(桂科产1123008-1);广西科技攻关项目(桂科攻1222009);广西农科院团队项目(桂农科2011YT01)

摘　　要：甘蔗宿根矮化病（Ratoon stunting disase,RSD）是甘蔗生产中最为严重的细菌病害之一，常导致感病品种新植蔗减产10％～15％，宿根蔗减产20％～25％，在干旱的情况下感病品种的宿根蔗产量损失可达60％。To produce the antiserum of ratoon stunting disease （RSD） of sugarcane, RSD bacteria were iso- lated from the cane juice of infected sugarcane plants using the improved SC medium, and then identified ac- cording to PCR detection results. ELISA was used for the titer determination, and Tissue Blot Immunoassay （TBIA） detection was done to compare the detection capacity between homedade RSD antiserums and the im- ported one from USA. The results showed that the isolated pathogen is similar to the reported Leifsonia xyli subsp, xyli （Lxx） in morphology. A specific PCR product with the molecular size of 438 bp was also ampli-fied from both bacteria. The ELISA titer of antiserum against Lxx was greater than 1：256 000. The TBIA detection results using homemade RSD antiserum were consistent with those detected by RSD antiserum from USA.

关 键 词：甘蔗宿根矮化病 多克隆抗体 制备 病菌 感病品种 细菌病害 甘蔗生产 产量损失 

分 类 号：S435.661[农业科学—农业昆虫与害虫防治]