nm23H1对肝癌细胞增殖及体内肿瘤形成能力的影响  

Effect of nm23H1 on proliferation, tumor formation and metastasis of hepatocarcinoma

作　　者：金嗣松[1] 潘星华[2] 王元和[1] 傅继梁[3] 胡以平[2] 

机构地区：[1]第二军医大学长征医院普外科,上海200433 [2]第二军医大学细胞生物学教研室 [3]第二军医大学医学遗传学教研室

出　　处：《中华肿瘤杂志》2000年第5期381-384,共4页Chinese Journal of Oncology

基　　金：国家自然科学基金资助项目(39500171)

摘　　要：目的　研究nm2 3H1对肝癌细胞增殖、体内肿瘤形成和转移的影响。方法　构建正反义nm2 3H1cDNA表达载体并转染肝癌细胞SMMC 772 1,得到nm2 3H1稳定最高和最低表达的两种细胞克隆 ,并进行细胞生长曲线测定、裸鼠皮下及脾包膜下移植试验。结果　转染反义表达载体后 ,肝癌细胞mRNA和蛋白表达下降 ,在体内、体外增殖加速 ;转染正义表达载体后 ,出现与之相反的结果。正义表达细胞接种组裸鼠肿瘤结节形成率、出现时间分别低于和高于反义表达细胞接种组。结论nm2 3H1基因可以抑制肝癌细胞SMMC 772 1的增殖、肿瘤形成和转移能力。Objective To study the effects of nm23H1 on in vitro proliferation, in vivo tumor formation and metastasis of hepatocarcinoma.Methods Vectors containing insert of either sense or antisense nm23H1 cDNA under the albumin enhancer/promoter for liver cell specific expression were constructed. The expression vectors were transfected into hepatocarcinoma cell line SMMC 7721. Stable cell clones with maximum and minimum expression of nm23 were obtained. Cell growth was measured in vitro and metastasis was observed in vivo by injecting cells into nude mice, subcutaneously or under the spleen capsule.Results In vitro, cell clone expressing antisense nm23H1 grew faster than that expressing sense nm23H1. In nude mice, the subcutaneously inoculated tumor grew faster and more aggressively in mice bearing antisense nm23H1 transfected hepatoma. So did metastasis of the antisense nm23H1 transfected hepatoma under the spleen capsule.Conclusion Apart from suppressing metastasis, nm23H1 significantly suppresses tumor cell proliferation in vitro and tumor growth in vivo.

关 键 词：肝肿瘤 肿瘤转移 NM23H1基因 

分 类 号：R735.705[医药卫生—肿瘤]