癌症患者外周血淋巴细胞银染核仁形成区变化规律研究  

Research on the regular pattern of Ag NOR changes in patients with cancer

作　　者：陈立奇[1] 万远廉[2] 丁丽华[3] 林其燧 刘广贤 李金翰[6] 陈家薇 陈克能[8] 

机构地区：[1]北京医科大学基础医学院,100083 [2]北京医科大学第一临床医院外科 [3]北京医科大学第三临床医院职业病中心 [4]中国医学科学院协和医院检验科 [5]解放军第三○七医院低温肿瘤研究室 [6]第一军医大学南方医院肿瘤科 [7]哈尔滨医科大学第二临床医院病理科 [8]北京市肿瘤医院

出　　处：《中华肿瘤杂志》2000年第5期401-402,共2页Chinese Journal of Oncology

摘　　要：目的　用核仁银染强度探讨肿瘤患者外周血Th淋巴细胞rDNA转录活性与机体免疫状况的关系。方法　应用计算机图像分析系统 ,对癌症、炎症、非癌非炎性疾病患者及正常人外周血Th(CD4+ )淋巴细胞中的核仁银染强度进行比较。结果　与炎症、非癌非炎症疾病患者及正常人相比 ,癌症患者Th淋巴细胞核仁银染强度显著下降。结论　癌症患者淋巴细胞rDNA转录活性可以灵敏地反映体内癌变进展程度与机体免疫状况的相互关系 ,有可能为临床诊断癌症提供一个简便可行的辅助方法。Objective To correlate the relationship between rDNA transcriptional activity of CD4 + helper T cells and immunity in patients with cancer through silver stainability of nucleolus organizing regions (Ag NORs).Methods The Th(CD4 +) rDNA transcriptional activity was compared among patients with cancer, infectious diseases, non cancerous non infectious diseases through image analysis.Results Th(CD4 +) rDNA transcriptional activity was decreased extremely in patients with cancer, but increased in patients with inflammation compared with normal controls.Conclusion The analysis of Th(CD4 +) rDNA transcriptional activity may serve as a new tumor marker for tumor diagnosis and differential diagnosis. Therefore, it may provide an innovative and reliable method for clinical cancer diagnosis.

关 键 词：癌症 外周血淋巴细胞 AGNOR染色 诊断 

分 类 号：R730.43[医药卫生—肿瘤]