酪氨酸-铒(Ⅲ)配合物与鲱鱼精DNA的作用机制  

Interaction Mechanism of Tyrosine-Erbium(Ⅲ) Complex and Herring Sperm DNA

作　　者：李志成[1] 王兴明[1] 赵娜[2] 

机构地区：[1]西南科技大学材料科学与工程学院,四川绵阳621010 [2]北京城市学院教务处,北京100083

出　　处：《中国稀土学报》2013年第2期142-147,共6页Journal of the Chinese Society of Rare Earths

基　　金：国家自然科学基金项目(30973634)资助

摘　　要：通过紫外-可见光谱法研究了酪氨酸(Tyr)与铒(Er,Ⅲ)的相互作用,结果表明:Er(Ⅲ)与Tyr可形成1∶3的配合物,该配合物的摩尔吸光系数为ε=6.19×104L.(mol.cm)-1;且研究了Er(Ⅲ)(Tyr)3与鲱鱼精DNA的相互作用,结果显示二者的作用摩尔比为2∶1,荧光探针法、黏度法和熔点法表明二者之间存在嵌插结合,Scatchard法表明二者之间既存在嵌插结合,又有一定的沟渠作用,圆二色法结果显示Er(Ⅲ)(Tyr)3可使DNA构像转变。The UV-Vis spectroscopy was used to study the interaction of tyrosine （Tyr） with the erbium （Er,IU ）. The results showed that the Er （Ⅲ） and Tyr could form a complex with the molar ratio of 1 ： 3, and its molar absorptivity was ε=6.19×104 L·（mol·cm）^-1 In addition, the interaction between Er（ Ⅲ ） （Tyr） 3 and DNA was studies. The results were as follows： they interacted with the molar ratio of 2： 1, and the fluorescence probe method, the viscosity method and the melting point method showed that the intercalation effects existed. The scatchard method also showed a weak groove effects, the circular dichroism method revealed that Er （ Ⅲ ） （Tyr）3 could made the change of conformation of DNA

关 键 词：酪氨酸(Tyr) 稀土配合物 鲱鱼精DNA 作用机制 

分 类 号：O629.74[理学—有机化学]