荧光原位杂交技术检测慢性B-CLL第12号染色体三体畸变  

DETECTION OF TRISOMY 12 IN CHRONIC B LYMPHOCYTIC LEUKEMIA (B CLL) BY FLUORESCENCE IN SITU HYBRIDIZATION

作　　者：周俊[1] 石为[1] 翁立红 胡晓彤 徐伟珍 顾文祥 张颜明 

机构地区：[1]浙江大学湖滨校区组胚教研室形态中心,杭州310006

出　　处：《肿瘤》2000年第5期349-351,共3页Tumor

基　　金：浙江省教委资助项目!编号 961063

摘　　要：目的　检测 2 0例慢性 B淋巴细胞白血病 (B-CL L )中第 12号染色体三体畸变。方法　运用荧光原位杂交技术 (FISH )。结果　 6例 (30 % )患者有 12号染色体三体 (+12 ) ,其中 5例经染色体核型分析发现的 + 12均得到证实。在 1例 B- CL L 中 ,染色体分析未发现任何分裂相 ,而 FISH检测发现了 + 12克隆。在另 1例 B- CL L 中 ,FISH不仅证实了染色体核型检查发现的存在于扁桃体的 + 12 ,同时还发现了染色体分析未发现的在外周血中的 + 12克隆。结论　本实验结果表明Objective To detect trisomy 12 in 20 patients with B CLL.Methods Using fluorescence in situ hybridization (FISH) technique with a centromeric probe for chromosome 12. Results Trisomy 12 was detected in 6 (30%) of 20 B CLL, and in 5/6 cases trisomy 12 revealed by chromosome karyotyping was confirmed by FISH study; in another case, chromosome analysis found no mitosis, while FISH identified a trisomy 12 clone still in another B CLL, cytogenetic study found +12 only in tonsil, while FISH not only proved +12 in tonsil but also in peripheral blood cells. Conclusion The study showed that FISH was more sensitive than traditional cytogenetics in detection of chromosome aberrations.

关 键 词：荧光原位杂交 慢性B淋巴细胞 白血病 三体畸变 

分 类 号：R733.72[医药卫生—肿瘤]