结肠癌转移相关基因1、肝细胞生长因子和C-met在子宫内膜癌中的表达及其意义  

Expression of MACC1,HGF and C-met in endometrial carcinoma and their clinical significance

作　　者：任淑敏[1] 史惠蓉[1] 张瑞涛[1] 陈志敏[1] 张明会[1] 

机构地区：[1]郑州大学第一附属医院妇产科,河南郑州450052

出　　处：《中国实用妇科与产科杂志》2013年第4期274-278,共5页Chinese Journal of Practical Gynecology and Obstetrics

摘　　要：目的探讨结肠癌转移相关基因1(metastasis-associated in colon cancer-1,MACC1)、肝细胞生长因子(HGF)和C-met蛋白在子宫内膜癌中的表达以及其相关的临床病理特点。方法应用免疫组织化学和RT-PCR技术检测2009年1月至2011年7月郑州大学第一附属医院妇产科行手术治疗的子宫内膜癌患者的癌变内膜标本39例及同期25例不典型增生的子宫内膜、25例单纯性增生子宫内膜、25例正常子宫内膜中MACC1、HGF和C-met蛋白、mRNA的表达情况,并分析3者与子宫内膜癌临床病理特征的关系及其相关性。结果 MACC1、HGF和C-met蛋白在子宫内膜癌组织中的阳性率分别为64.1%、61.5%和69.2%,显著高于正常、单纯增生以及不典型增生的内膜组织,差异均有统计学意义(P<0.05)。子宫内膜癌中MACC1、HGF、MET基因的mRNA相对表达量分别为0.216±0.062、0.134±0.054、0.172±0.044,也明显高于其他3组,差异均有统计学意义(P<0.05)。MACC1、HGF和C-met蛋白的异常高表达与子宫内膜癌的临床分期、肌层浸润和淋巴结转移相关,临床分期越晚、肌层浸润越深、伴随淋巴结转移的癌组织中MACC1、HGF和C-met蛋白表达越高(P<0.05)。子宫内膜癌组织中MACC1蛋白的表达与HGF和C-met蛋白的表达呈正相关(r=0.525,P=0.000;r=0.393,P=0.008),HGF蛋白与C-met蛋白表达呈正相关(r=0.334,P=0.027)。结论在子宫内膜癌组织中MACC1具有作为子宫内膜癌诊断、治疗评估分子标志物的潜在价值,MACC1、HGF和C-met蛋白表达异常可能协同参与子宫内膜癌的进展。Objective To investigate the expression of MACC 1, HGF, and C-met in endometrial carcinoma and their clinical significance. Methods The expressions of MACC1, HGF and C-met in 25 specimens of normal endometrium tissues,25 specimens of simple hyperplasia endometrium tissues,25 specimens of atypical hyperplasia endo -metrium tissues, and 39 specimens of endometrial carcinoma were measured by immunohistochemistry and reverse transcription-polymerase chain reaction （ RT- PCR）. The correlations of the expressions of MACC1, HGF and C-met to the clinicopathologic characteristics of endometrial carcinoma were analyzed, and the correlations between the expressions of the 3 proteins were also evaluated. Results The positivity rates of MACC1 ,HGF and C-met proteins were 64. 1% ,61.5% ,and 69. 2% in endometrial carcinoma with relative mRNA expressions of 0. 216±0. 062,0. 134±0. 054,0. 172± 0. 044, showing significant differences from those in normal endometrium tissues, simple hyperplasia endometrium tissues, and atypical hyperplasia endometrium tissues（P 〈 0. 05 ）. In endometrial carcinoma, the up-regulation of MACC 1, HGF and C-met expressions were associated with advanced FIGO stage, muscle layer infiltration and lymph node metastasis （ P 〈 0. 05）. MACC1 expression was positively correlated to HGF （ r = 0. 525 ,P = 0. 000） and C-met expressions （ r = 0. 393, P = 0. 008 ）, and the latter two was also positively correlated （r = 0. 334,P = 0. 027）. Conclusion MACC1 may serve as a potential biomarker for advanced endometrial carcinoma. Deregulation of MACC1, HGF and C-met proteins may synergistically partici- pate in the malignant progression of endometrial carcinoma.

关 键 词：子宫肿瘤 结肠癌转移相关基因1 肝细胞生长因子 C-MET 

分 类 号：R737.3[医药卫生—肿瘤]