重离子和X射线对人微血管内皮细胞血管生成的抑制作用  

Inhibitory Effect of ^(12)C^(6+) Ion and X-ray on Angiogenesis in HMEC-1

作　　者：刘圆圆[1,2,3] 张红[1,2,3] 刘阳[1,2,3] 孙超[1,2,3,4] 武振华[1,2,3] 李鸿岩[1,2,3,4] 

机构地区：[1]中国科学院近代物理研究所,甘肃兰州730000 [2]中国科学院重离子束辐射生物医学重点实验室,甘肃兰州730000 [3]甘肃省重离子束辐射医学应用基础重点实验室,甘肃兰州730000 [4]中国科学院大学,北京100049

出　　处：《原子核物理评论》2013年第1期84-88,共5页Nuclear Physics Review

基　　金：国家重点基础研究发展计划项目(973计划)(2010CB834202);国家自然科学基金重点项目(10835011);国家自然科学基金面上项目(10675151);中国科学院西部之光人才培养计划项目(XB106012)~~

摘　　要：研究了X射线和重离子照射对人微血管内皮细胞的增殖、迁移、管样结构形成及基质金属蛋白酶表达的影响。Transwell迁移实验结果显示,照射24 h后,非毒性照射剂量照射能抑制HMEC-1细胞的迁移,且重离子照射抑制内皮细胞迁移能力较X射线的强。基质胶实验显示,重离子照射可显著抑制自发性管样结构的形成,但X射线照射的抑制作用不明显。同时,明胶酶谱法证实了重离子照射可明显抑制HMEC-1细胞中基质金属蛋白酶(MMP-2和MMP-9)的表达,而X射线照射对此类酶几乎无任何影响,显示了重离子较X射线的肿瘤放疗优势所在。The study aims to explore the effect of 12^C^6＋ ion and X-ray on proliferation, migration, tube formation and MMPS of human microvascular endothelial cells （HMEC-1 ）. Transwell migration experiment showed that the radiation could inhibit the migration of HMEC-1 in sub-lethal dose 24 h after irradiation. Furthermore, the inhibition ability of 12^C^6＋ ion was stronger than that of X-ray. Matrigel experiment indicated that 12^C^6＋ ion suppressed the tube formation of HMEC-1 spontaneously. However, the inhibitory effect of X-ray had no significance. Meanwhile, Gelatin Zymography showed the expression of MMP-2 was inhibited obviously by 12^C^6＋ ion, while X-ray had little effect on the expression of MMP-2. In conclusion, 12^C^6＋ ion is superior to X-ray in radiotherapy of tumor.

关 键 词：微血管内皮细胞 重离子照射 抑制作用 血管生成 基质金属蛋白酶 X射线照射 迁移能力 线对 

分 类 号：R730[医药卫生—肿瘤] R730.5[医药卫生—临床医学]