SEA对PLA801D细胞体外生物学行为影响及相关机制  

The effect of SEA on PLA801D cell biologic behaviors in Vitro and its related mechanism

作　　者：王力玄[1] 任锦[2] 吴艳峰[2] 周佳莹[2] 

机构地区：[1]吉林大学白求恩医学院,09级临床医学专业吉林长春130021 [2]吉林大学第二医院呼吸内科,吉林长春130041

出　　处：《中国实验诊断学》2013年第3期442-444,共3页Chinese Journal of Laboratory Diagnosis

摘　　要：目的探讨葡萄球菌肠毒素A(stap-hylococcal enterotoxins,SEA)对人肺巨细胞癌株(PLA801D)细胞生长、增殖、凋亡等生物学行为的影响,为人肺癌的生物治疗提供实验依据。方法 MTT比色法检测不同浓度的SEA对PLA801D细胞的增殖抑制率,流式细胞仪检测细胞周期进程的变化,透射电子显微镜检测PLA801D亚细胞水平改变。结果不同浓度的SEA对PLA801D细胞增殖抑制率分别为22.8%、33.7%和42.1%,与对照组相比差异显著。流式细胞仪结果显示G0/G1期细胞增多,S期细胞减少,G2/M期细胞相对增多,电子显微镜下见PLA801D细胞膜破裂,线粒体肿胀,可见凋亡小体改变。结论 SEA能显著抑制PLA801D细胞的增殖抑制率,抑制G1期向S期细胞转化进程,诱导PLA801D细胞凋亡及亚细胞结构改变。Objective Discuss the effect of SEA on PLA801D cell biologic behaviors including the cell growth, pro- liferation and apoptosis in Vitro,to provide experimental basis for biological treatment for human lung cancer. Methods The proliferation inhibition ratio of SEA in different concentrations on PLA801D cells were tested by MTT, the changes of cell cycle progression was assayed with flow cytometry,and the changes of PLA801D cells in subcellular lev- el were investigated by transmission electron microscopy. Results The proliferation inhibition ratio of SEA in different concentrations on PLA80iD were 22.8%,33.7% and 42.1% respectively, and the results were significantly different from the control group. The results of flow eytometry showed the cells in G0/G1 phase increase,the cells in S phase de- crease,and the cells in G2/M phase relatively increase. Under electron microscopy, there are membrane rapture, mito- ebondria swelling and apoptotie body in PLA801D cells. Conclusion SEA can significantly inhibit the proliferation inhibition ratio of PLA801D cells,inhibit the transformation of cells from G1 phase to S phase,and induce the apoptosis and subcellular structure changes of PLA801D cells.

关 键 词：PLA801D细胞株 SEA 细胞周期 凋亡 

分 类 号：R734.2[医药卫生—肿瘤]