微胶囊剂型黄芪多糖对刺参生长性能、免疫力及抗病力的影响  

Impact of APS microcapsules on growth,immunity and disease resistance of sea cucumber Apostichopus japonicus

作　　者：樊英[1] 王淑娴[1] 李天保[1] 许拉[1] 于晓清[1] 叶海斌[1] 刁菁[1] 王勇强[1] 

机构地区：[1]山东省海水养殖研究所病害防治重点实验室,青岛266002

出　　处：《渔业科学进展》2013年第1期119-125,共7页Progress in Fishery Sciences

基　　金：山东省农业重大应用技术创新课题(鲁财农指[2010]58号);海洋公益性行业科研专项经费项目200905020;山东省科技发展计划项目(2010GHY10501);山东省优秀中青年科学家科研奖励基金项目(BS2010NY021)共同资助

摘　　要：在基础饲料中添加不同剂量(1%、3%、5%)的微胶囊剂型黄芪多糖(Astragalus polysac-charudes,APS)(载药量为17%),制成3种配合饲料,连续投喂初始体重为22.0±1.0g的刺参Apostichopus japonicus35d,探讨微胶囊剂型黄芪多糖(以下简称"微胶囊")对刺参生长性能、非特异性免疫及抗病力的影响。结果显示,35d后刺参最高特定生长率(Specific growth rate,SGR)为0.51%/d(5%添加组)(P<0.05);微胶囊添加量为3%时免疫效果最佳,刺参体腔液中酸性磷酸酶ACP、超氧化物歧化酶SOD、髓过氧化物酶MPO活性显著高于对照组(P<0.05)。试验期间每隔7d测定刺参体腔液样品,其中ACP活性在第28天最高,为11.2U/100ml(P<0.05);SOD活性在第21天最高,为63.3U/ml(P<0.05);MPO活性在第21天最高,为40MPO单位/ml(P>0.05);免疫试验结束后对刺参进行灿烂弧菌Vibrio splendidus攻毒实验,14d内观察得出,3%微胶囊添加组累积死亡率明显低于对照组(P<0.05)。由此可得,微胶囊剂型黄芪多糖对仿刺参生长性能、免疫力及抗病力方面均具有显著的增强效果,以3%添加量效果最佳,且添加量、作用时间、免疫效果之间存在相关性。The study was conducted to investigate the impact of Astragalus polysaccgarudes（APS）microcapsules（drug loading 17%）on the growth,nonspecific immune indices and resistance of Apostichopus japonicas against Vibrio splendidus infection.APS microcapsules were added at graded levels（1%,3%,and 5%）to the basal diet to formulate three practical compound diets.Continuous 35-day feeding trials were carried out on A.japonicus at initial body weight of 22.0±1.0g.After feeding the diets for 35days,specific growth rate（SGR）for the 5%treatment was 0.51% /d,which was significantly higher than the control.The activities of serum acid phosphatase（ACP）,superoxide dismutase（SOD）,and myeloperoxidase（MPO）increased significantly（P〈0.05）in the 3% treatment.During the experiment these factors were determined at 7-day intervals.The activity of ACP was 11.2U /100ml at 28dduring the experiment（P〈0.05）,the activity of SOD was 63.3U /ml at 21d（P〈0.05）,and the activity of MPO was 40MPO unit /ml at 21d（P〈0.05）.After the feeding trial,aV.splendidusinjection challenge was given to the sea cucumbers to test the effects of APS microcapsule.The 3% treatment showed significantly better survival than the control（P〈0.05）.It is suggested that supplementation of 3% APS microcapsules has the greatest effects on growth,immunity and disease resistance in A.japonicas against V.splendidus.In addition,there was a direct relationship between the level and time of treatment,and the immunity.

关 键 词：微胶囊 黄芪多糖 刺参 生长 免疫 抗病力 

分 类 号：S963[农业科学—水产养殖]