不同植物材料中SOD的初步纯化及性质  

Primary purification and properties of superoxide dismutase from different plants

作　　者：王俊英[1] 

机构地区：[1]周口师范学院生命科学系,河南周口466001

出　　处：《周口师范学院学报》2013年第2期76-78,95,共4页Journal of Zhoukou Normal University

摘　　要：以大蒜、芦荟为实验材料,采用热变性结合硫酸铵分段盐析法进行SOD的初步纯化,并对其SOD的部分性质做了研究.结果表明:大蒜和芦荟SOD的最终纯化倍数分别为6.2和3.3,活力回收率分别为55%和46%,最适温度分别为40℃和45℃,最适pH值分别为7.0和9.0.两者SOD均有较好的热稳定性.Using garlic, aloe vera as experimental material, adopt thermal denaturation and then the pieeewise ammonium sulfate salting - out law method to preliminarily purify SOD, besides, part of the nature of SOD was studied. The results showed that the eventually purification multiples of garlic and aloe SOD were respectively 6.2and 3.3, activity recovery of SOD were respectively 55% and 46%, and the optimum temperature were respectively 40 ℃ and 45 ℃, the optimal pH value were respectively 7.0 and 9.0. Both SOD had good thermal stability.

关 键 词：大蒜 芦荟 超氧化物歧化酶 初步纯化 性质 

分 类 号：TQ914[化学工程]