RFLP、RAPD、AFLP在水稻农垦58S和1514中多态性比较  

Comparison of Polymorphism of RFLP, RAPD and AFLP in Rice Nongken 58S and 1514

作　　者：陈亮[1] 梅明华[2] 徐才国 李子银 陈睦传[1] 沈明山[1] 

机构地区：[1]厦门大学细胞生物学与肿瘤细胞工程教育部重点实验室,厦门361005 [2]华中农业大学作物遗传改良国家重点实验室,武汉430070 [3]中国科学院遗传研究所,北京100101

出　　处：《植物学通报》2000年第5期424-428,共5页Chinese Bulletin of Botany

摘　　要：本文用RFLP、RAPD和AFLP三种分子标记技术对农垦 58SX1 51 4组合及其F2 极性集团进行了分析 ,比较了它们多态性和阳性的比率 ,结果显示 ,三种分子标记的多态性和与目的基因连锁的阳性比率分别为 1 9.93% ,5.2 3% ;1 1 .1 7% ,0 .76%和 86.47% ,7.52 %。AFLP的多态性比率和阳性比率均为最高。RFLP, RAPD and AFLP analysis were performed between Nongken 58S and 1514, two varieties of rice, and their bulked extremes of F 2. The rate of polymorphism and positive marker linked to target gene was compared. The results indicated that the rates of polymorphism and positive marker of RFLP, RAPD and AFLP are 19.93%, 5.23%;11.17%, 0.76% and 86.47%, 7.52%, respectively. The rate of polymorphism and positive marker of AFLP is highest in the three kinds of molecular marker systems. It was discussed that the advantages and disadvantages, and application of the three kinds of molecular marker systems in constructing a high resolution map of a specific chromosome region and selecting linkage markers to target gene.

关 键 词：RFLP RAPD AFLP 水稻 多态性比率 阳性标记比率 

分 类 号：Q943[生物学—植物学]