细胞红蛋白基因在新生大鼠缺氧缺血性脑损伤时的表达  

Expression of cytoglobin gene in hypoxic-ischemic brain damage of newborn rat

作　　者：杨汉华[1] 田树凤[1] 史雪川[1] 肖丹萍[1] 

机构地区：[1]汕头大学医学院第二附属医院儿科,515041

出　　处：《国际儿科学杂志》2013年第2期208-210,共3页International Journal of Pediatrics

基　　金：广东省医学科研基金立项（B2009180）;广东省科技计划项目（20088080701036）

摘　　要：目的探讨缺氧缺血性脑损伤时脑中细胞红蛋白基因的表达及变化。方法将出生7d的SD大鼠50只分成5组，其中实验组在手术后制成脑损伤模型，并且在血流阻断4、12、24、48h取脑；对照组为假手术组，术后即取脑。设计细胞红蛋白基因引物，提取脑组织总RNA，进行PCR。以GraphPadPrism统计软件包的One-way单因素方差分析进行数据统计分析。结果设计的新生大鼠细胞红蛋白cDNA序列引物可靠，扩增产物与设计相符。实验组细胞红蛋白mRNA的表达在缺血4h开始升高，24h达到高峰，48h开始下降，与对照组相比，差异有统计学意义（P〈0．01）。结论新生大鼠缺氧缺血性脑损伤后细胞红蛋白mRNA表达增强，提示细胞红蛋白可能在脑缺氧的适应性调节过程中起重要作用。Objective To investigate the expression and change of cytoglobin（Cygb）gene on hypoxicischemic brain damage（HIBD）. Methods Fifty SD rats of 7days old were divided into four study groups and one control group. The brain tissues were taken at 4hours, 12hours ,24hours and 48hours after the onset of HIBD. Cygb mRNA was determined by the reverse transcription PCR. One-way method of GraphPad Prism was used for statistics. Results The fragment length of PCR products was identical with experimental design. The expression level of Cygb gene increased at 4h after ischemia,and peaked at 24h. 48h after HIBD,the Cygb gene level began to decrease. Conclusion The expression of Cygb in brain tissue increased rapidly after HIBD of newborn rats. suggesting that Cwb may have important function in the protection process of HIBD.

关 键 词：细胞红蛋白 新生大鼠 基因表达 缺氧缺血性脑损伤 

分 类 号：R742[医药卫生—神经病学与精神病学]