大鼠延髓背角Ⅱ层神经元向臂旁区、延髓尾侧腹外侧区和脊髓的投射  

THE PROJECTIONS FROM LAMINA Ⅱ OF MEDULLARY DORSAL HORN TO PARABRACHIAL REGION, CAUDAL VENTROLATERAL MEDULLA AND SPINAL CORD IN THE RAT

作　　者：武建宏[1] 李云庆[1] 施际武[1] 

机构地区：[1]第四军医大学基础部解剖学教研室,梁銶琚脑研究中心西安710032

出　　处：《神经解剖学杂志》2000年第3期251-256,共6页Chinese Journal of Neuroanatomy

基　　金：国家杰出青年科学基金资助项目!( No. 3 962 5 0 11)

摘　　要：将逆行追踪剂荧光金分别注入大鼠一侧臂旁区、延髓尾侧腹外侧区和颈髓第四节段 ,观察到延髓背角浅层 ( 、 层 )向上述部位均有投射 , 层外侧部的投射神经元多于 层内侧部。臂旁区接受双侧延髓背角浅层的投射 ,但以同侧为主 ;延髓尾侧腹外侧区和第 4颈髓接受同侧延髓背角浅层神经元的投射但延髓背角浅层向第 4颈髓投射的神经元数量较少。结合免疫荧光组织化学研究表明 , 层向臂旁区和延髓尾侧腹外侧区投射的神经元部分呈 calbindin-D2 8k样阳性 ,而未见呈 parvalbumin样阳性者。向第 4颈髓投射的 层神经元数量很少 ,未见有呈 calbindin-D2 8K和 parvalbumin样阳性者。本研究结果进一步支持 层有传出投射的观点 ,也提示 层的 calbindin-D2 8K样和 parvalbumin样阳性神经元可能分别属于不同的细胞亚群。After injecting retrograde tracer fluoro gold(FG) into the parabrachial region(PB), caudal ventrolateral medulla(CVLM) and the fourth segment of cervical spinal cord (C 4), respectively, neurons in laminae Ⅰ～Ⅱ of the medullary dorsal horn projecting to the above mentioned brain areas were observed. PB received projections from bilateral laminae Ⅰ and Ⅱ with an ipsilateral dominance; CVLM and C 4 received projections from ipsilateral laminae Ⅰ and Ⅱ. Neurons projecting to C 4 were very sparsely distributed in laminae Ⅰ and Ⅱ of the medullary dorsal horn. The projecting neurons in outer part of lamina Ⅱ were more than those in inner part of lamina Ⅱ. Combined with immunofluorescence histochemistry for calbindin D28k(CB) and parvalbumin(PV), it was demonstrated that a part of neurons projecting to PB or CVLM showed CB like immunoreactivity, but none of them exhibited PV like immunoreactivity. There were only a few neurons in lamina Ⅱ projecting to C 4 and they exhibited neither CB nor PV like immunoreactivity. The present study provides further evidence for the existence of projecting neurons in lamina Ⅱ and suggests that immunostaining against CB and PV may distinguish two neuronal subpopulations in lamina Ⅱ.

关 键 词：荧光金 延髓背角Ⅱ层 PB CVLM C4 投射神经元 

分 类 号：R741.02[医药卫生—神经病学与精神病学]