鼻咽癌恶性转化基因Tx中3.0kb片段序列分析  

Nucleotide Sequence Analysis of XhoⅠ/EcoRⅠ 3.0 kb Fragment from a Transforming Gene Tx of Human Nasopharyngeal Carcinoma Cell Line CNE2

作　　者：黎明[1] 任维[1] 廖伟[1] 翁新宪[1] 夏林庆[1] 曹亚[1] 

机构地区：[1]湖南医科大学肿瘤研究所,长沙410078

出　　处：《中国生物化学与分子生物学报》2000年第5期602-605,共4页Chinese Journal of Biochemistry and Molecular Biology

基　　金：中华医学基金!( CMB9665 5 );国家自然科学资金重点项目!( 3 983 0 410 )资助

摘　　要：从中国人鼻咽癌细胞株 CNE2中克隆分离出的恶性转化基因 Tx,其基因长度为 1 6kb.在对其中 2 .8kb片段测序的基础上 ,对其中 Xho /Eco R 长度约 3.0 kb的片段 ( Tx3.0 )进一步进行了测序 ,并利用生物信息学技术分析认为 ,Tx3.0与人类免疫球蛋白 kappa( Igκ)轻链基因高度同源 ,并直接映射于 J区 .Tx3.0中除有编码免疫球蛋白 kappa链的 J2、J3、J4及 J5基因片段外 ,在各个片段间不仅有 TATA box、CAAT box和 Poly A等经典的调控序列 ,还有 NF- IL6的反应元件、某些转录因子的识别序列、以及核基质结合序列等 .据此以及 2 .8kb序列的分析结果 ,对 Tx3.0下游 1 .0 kb片段序列进行了预测 .对 Tx3.0基因片段的研究为进一步研究 Tx基因在鼻咽癌发病中的作用 ,提供了重要信息 .Recently Cao Ya et al have reported that a new transforming gene,designated Tx gene,was isolated from human nasopharyngeal carcinoma(NPC) cell line CNE2.The previous data indicated that this gene was different from a number of known oncogene,and played a role in NPC carcinogenesis.The 2 8 kb Eco RⅠ/ Eco RⅠ fragment of Tx gene showed that it was highly homologous with Ig kappa constant region.Based on the restriction map of Tx gene,another Xho Ⅰ/ Eco RⅠ Tx 3 0 fragment was sequenced and analyzed by bioinformatics.This fragment contained Igκ J2,J3,J4 and J5 fragments,an N segment and several RSSs.Therefore,Tx3.0 was highly homologous with human Igκ gene and was in agreement with its J region.Besides classic canonical TATA boxes and CAAT boxes,Tx3.0 fragment also contained several NF IL6 binding sites,transcription factors binding sites and its 3′ terminal region contained a part of MAR.The identification of these elements and their precise localization in Tx3.0 are significant for further elucidating the role of Tx gene in carcinogenesis of NPC.

关 键 词：鼻咽癌 Tx基因 3.0kb片段 序列分析 发病机制 

分 类 号：R739.630.2[医药卫生—肿瘤] Q78[医药卫生—临床医学]