sox19b在斑马鱼胚胎眼睛发育中的作用  

Effect of sox19b gene knockdown on the eyes development in zebrafish

作　　者：高明[1] 杨阳[1] 陈学冉[1] 史玮[1] 林浴霜[1] 郝爱军[1] 

机构地区：[1]山东大学医学院组织学胚胎学教研室、教育部实验畸形学重点实验室,济南250012

出　　处：《解剖学报》2013年第2期264-268,共5页Acta Anatomica Sinica

基　　金：国家自然科学基金资助项目(81071057);教育部高等学校博士学科点专项科研基金资助项目(20110131110036)

摘　　要：目的通过抑制sox19b基因的表达,探讨sox19b基因在斑马鱼胚胎眼睛发育和形成中的作用。方法通过显微注射sox19b吗啉寡聚核苷酸(MO)抑制sox19b基因的表达,观察胚胎发育过程中表型的变化;采用石蜡包埋组织切片及HE染色、RT-PCR和整封原位杂交等方法探讨敲除sox19b后对斑马鱼胚胎眼睛发育的调控机制。结果敲除sox19b基因后,斑马鱼胚胎眼睛发育受到影响,表现为眼睛变小或缺失,视网膜及晶状体结构发育异常(n=57/93);眼睛发育过程中重要调控基因rx3、pax2a及vsx2等表达明显降低,进而影响眼睛的发育和形成。结论 sox19b基因作为转录调控因子,可以通过调节眼区转录因子的表达进而影响斑马鱼胚胎早期眼睛的发育和形成。Objective To explore the function of sox19b on the eye development in zebrafish by knockdown of soxlgb. Methods To inhibit the expression of sox19b by mieroinjection of sox19b morpholinos（MO）and then observe the effect of the development of the eyes in zebrafish by RT-PCR and the whole-mount in situ hybridization. Results The development of the eyes was abnormal after knockdown of sox19b, mainly showed small eyes or eye deficiency and the abnormal retina and lens（ n = 57/93 ）. The expression of some important genes during the development of the eyes, such as rx3, pax2a and vsx2, was decreased apparently in the soxlgb morphant. Conclusion The transcription factor, soxlgb is critical for the development of the eyes via the regulation of the important eye-field transcription factors, EFTEs in early zebrafish embryos.

关 键 词：sox19b 吗啉寡聚核苷酸 眼睛发育 眼区转录因子 原位杂交 反转录-聚合酶链反应 斑马鱼 

分 类 号：R394.1[医药卫生—医学遗传学]