人类多重剪接RNA结合蛋白类基因的克隆和分析  

Clone and analysis of human RNA binding protein with multiple splicing 2 gene

作　　者：张雅娟[1] 肖娟娟[2] 鲁德意[2] 王尉平[2] 

机构地区：[1]苏州卫生职业技术学院检验药学系,江苏省215009 [2]苏州大学医学部基础医学与生物科学学院生物化学与分子生物学教研室

出　　处：《江苏医药》2013年第6期624-628,共5页Jiangsu Medical Journal

摘　　要：目的通过同源筛选的方法拼接并克隆人类多重剪接RNA结合蛋白2(RBPMS2)基因。方法采用同源筛选策略,以人RBPMS2基因作为信息探针,在GeneBank数据库中进行分析,将获得的高度同源的表达序列标签(EST)用VECTOR NTI软件拼接成重叠群。通过UCSCGenome Blat Server分析,确定RBPMS2基因的染色体定位,SMART网上分析工具进行结构域预测,Unigene数据库进行表达谱分析。结果电子克隆到人类RBPMS2新基因cDNA序列,含完整的开放阅读框(ORF)。RBPMS2基因编码的多肽由209个氨基酸组成,分子量22.5KDa,等电点8.63。SMART分析显示RBPMS2蛋白包含1个RNA识别模体(RRM)结构域。RBPMS2基因位于第15号染色体,定位在15q22.31,由7个外显子和6个内含子组成。电子表达谱分析显示RBPMS2在卵细胞、受精卵、囊胚、不同发育阶段的胚胎、膀胱、肾脏等组织中高表达。结论分析并克隆到了一个新的人类RBPMS2基因。Objective To assemble and clone human RNA binding protein with multiple splicing 2 （RBPMS2） gene by homologous sequence aligning. Methods Human RBPMS2 gene wastaken as an information probe for searching the coding sequece in GeneBank database by homologous sequence aligning. Then the highly homologous expressed sequence tag（EST） was assembled to thecontig using VECTOR NTI software. The chromosome localization, domain and expression pattern of RBPMS2 were analyzed by UCSC Genome Blat Server, SMART and Unigene database, respectively.Results A novel cDNA sequence o{ human RBPMS2 gene was cloned,which contained complete open reading frame（ORF）. RBPMS2 gene encoded a 209 amino acides protein with MW 22.5 KDa and pI8.63. SMART analysis showed that RBPMS2 protein included a RNA recognition motif （RRM） domain. RBPMS2 was mapping on the fifteenth chromosome, located at 15q22.31, and consisted ofseven exons and six introns. Unigene database presented that RBPMS2 was widely expressed in ovotid, zygote, blastula, embryo at different developmental stages, bladder and kidney. Conclusion Anovel human RBPMS2 gene has been cloned and analyzed.

关 键 词：多重剪接RNA结合蛋白 克隆 

分 类 号：R735[医药卫生—肿瘤]