非白色假丝酵母菌代谢产物对人脐静脉内皮细胞株ECV304细胞增殖的诱导作用  

Effects of non-Saccharomyces albicans metabolic products on the proliferation of human umbilical vein endothelial cell ECV304

作　　者：陈斌[1] 车团结[2] 白德成[3] 何祥一[1] 

机构地区：[1]兰州大学口腔医学院口腔修复学教研室 [2]兰州大学生命科学院细胞与分子生物学实验室 [3]兰州大学基础医学院甘肃省新药临床前研究重点实验室,兰州730000

出　　处：《华西口腔医学杂志》2013年第2期186-190,共5页West China Journal of Stomatology

基　　金：中央高校基本科研业务费专项基金资助项目(lzujbky-2010-142)

摘　　要：目的研究3种常见的非白色假丝酵母菌对人脐静脉内皮细胞株ECV304细胞增殖及细胞周期的影响。方法制备热带假丝酵母菌、克鲁斯假丝酵母菌和光滑假丝酵母菌上清液并行倍比稀释,设1、4、16倍3个稀释度以及对照组。体外培养ECV304细胞,分别将3种非白色假丝酵母菌不同稀释度的上清液与ECV304细胞共培养,采用MTT法测定培养24、48、72 h后的细胞增殖率;倒置显微镜观察培养48 h后细胞密度的变化;流式细胞术测定培养48 h后对ECV304细胞周期的影响。结果培养24 h后,3种假丝酵母菌1倍稀释的上清液均可促进细胞增殖,4倍稀释的克鲁斯假丝酵母菌也可促进细胞增殖(P<0.05);培养48、72 h后,克鲁斯假丝酵母菌和光滑假丝酵母菌上清液仍可促进ECV304细胞增殖,而热带假丝酵母菌上清液无论稀释度高低均不能促进ECV304细胞增殖。培养48 h后,克鲁斯假丝酵母菌和光滑假丝酵母菌上清液组的细胞密度明显增高,同时其G0/G1期细胞所占比例降低,细胞增殖指数(PI)升高(P<0.05);而热带假丝酵母菌组的细胞密度和PI均无明显变化(P>0.05)。结论克鲁斯假丝酵母菌和光滑假丝酵母菌的代谢产物对ECV304细胞的增殖有诱导作用,临床上应加强非白色假丝酵母菌感染的检测和治疗。Objective To evaluate the effects of non-Saccharomyces a!bicans metabolic products on the cell cycle distribution and proliferation of human umbilical vein endothelial cell ECV304 cells in vitro. Methods The parallel dilution supernatant of Saccharomyces tropicalis, Saccharomyces krusei and Saccharomyces glabrata were prepared, and 1, 4, 16-fold（s） diluted concentration and control group were set up. The line of human umbilical vein endothelial cell ECV304 was cultured in vitro and treated by non-Saccharomyces albicans supernatant. The proliferous effect of ECV304 induced by non-Saccharomyces albicans supernatant after 24, 48, 72 h was detected by the methods of MTI＇, and the changes of cell density and cycle after 48 h were investigated by inverted microscope and flow cytometry. Results At the 24th hour, all of the higher concentration （1-fold） of non-Saccharomyces albicans supernatant and the 4-folds diluted Saccharomyces krusei could promote ECV304 proliferation（P〈0.05）. After adding various non-Saccharo- myces albicans supernatant at 48th and 72th hour, Saccharomyces krusei supernatant and Saccharomyces glabrata supernatant significantly increased proliferation rate of ECV304, while Saccharomyces tropicalis supernatant group showed no significant change no matter which concentration was tested. At 48th hour after adding the non-Saccharomyces al bicans supernatant, the ECV304 cells density treated by Saccharomyces krusei supernatant and Saccharomyces glabrata supernatant were significantly higher under the inverted microscope. The G0/G1 population of ECV304 cells decreasedwhile cell proliferation index （PI） increased alter incubated with Saccharomyces krusei supernatant and Saccharomyces glabrata supernatant for 48 hours （/9〈0.05）. Saccharomyces tropicalis group showed no significant change （P〉O.05）. Conclusion The metabolic products of Saccharomyces krusei and Saccharomyces glabrata couldinduce proliferation of ECV304 cell, which suggests non-Saccharomyces albicans should be u

关 键 词：ECV304细胞 非白色假丝酵母菌 增殖 代谢产物 

分 类 号：R780.2[医药卫生—口腔医学]