醋酸棉酚对人舌鳞癌Tca8113细胞增殖的影响  

Inhibitary Effects of Gossypol Acetic Acid on Human Tongue Carcinoma Cell Line Tca8113

作　　者：付帅[1] 彭庆芳[1] 陈文飞[1] 吴勇[1] 

机构地区：[1]昆明医科大学附属口腔医院口腔颌面外科,云南昆明650031

出　　处：《口腔医学研究》2013年第3期210-212,216,共4页Journal of Oral Science Research

基　　金：国家自然科学基金资助项目(编号:81160326);云南省科技计划项目(编号:2009CD207)

摘　　要：目的:研究醋酸棉酚(gossypol acetic acid,GAA)对人舌鳞癌Tca8113细胞生长和凋亡的影响。方法:应用四唑盐比色法(MTT)及流式细胞术(FCM)观察GAA对人舌癌Tca8113细胞形态变化、增殖以及细胞凋亡的作用。结果:MTT法检测显示,作用24～72h,GAA对人舌鳞癌Tca8113细胞生长具有抑制作用;FCM检测结果显示30μmol/L的GAA作用48h、15μmol/L的GAA作用72h后,细胞凋亡率均较对照组升高(P<0.05)。结论:GAA能抑制人舌鳞癌Tca8113的生长并诱导细胞凋亡。Objective： To study the effects of gossypol acetic acid （GAA）on proliferation and apoptosis of human tongue carcinoma cell line Tca8113. Methods： MTT assay and FCM were used to study the effect of GAA on the proliferation and apoptosis of human tongue carcinoma cell line Tca8113. Results： Experimental GAA group could be observed on proliferation inhibition of Tca8113 cells after being treated by GAA for 24h to 72h through MTT assay. Compared with the control group, the result of FCM assay indicated that GAA （309mol/L GAA for 48h and 159mol/L for 72h） induced a higher apoptosis rate of TcaSll3 cells（P〈0.05）. Conclusion： GAA has inhibitory effects on the proliferation and induces apoptosis of human tongue carcinoma cell line Tca8113.

关 键 词：人舌鳞癌棉酚细胞培养 流式细胞术 

分 类 号：R739.8[医药卫生—肿瘤]