不同低功率He:Ne激光对种植体骨结合影响的实验研究  

Study on Effect of Different Low-power Laser Irradiation on Osseointegration of Dental Implants

作　　者：吕聪 徐世同[2] 段瑞平 

机构地区：[1]烟台市口腔医院种植科,山东烟台264008 [2]广东省口腔医院 [3]昆明市口腔医院

出　　处：《口腔医学研究》2013年第3期224-227,232,共5页Journal of Oral Science Research

基　　金：广州市海珠区科技重点攻关项目(编号:2007-Y-005)

摘　　要：目的:探讨不同低功率激光照射对种植体-骨结合界面骨形成的影响。方法:成年雄性Beagle犬6只,每侧胫骨植入4枚种植体,种植体随机编入A、B、C组实验组,D组对照组。实验组术后立即采用不同功率He:Ne激光垂直照射种植体对应体表,A组15mW、B组25mW、C组35mW,20min/次,1次/d,持续2周。术后1、2、3个月分别处死2只犬,处死前2周行四环素荧光双标记,制作带种植体的骨磨片,荧光正置显微镜下,计算双荧光标记率、矿化沉积率。Goldner’s三色法染色后测量种植体骨接触率。结果:骨组织形态计量学分析:术后1个月A、B组荧光标记率、矿化沉积率显著高于空白对照组及C组。A、B组间及空白对照组与C组间无显著差异。术后2、3个月荧光标记率、矿化沉积率各组间均无显著差异。组织形态学分析:种植体骨接触率术后1、2个月各组间无显著差异,术后3个月A、B组明显高于C、D组。结论:种植术后持续照射低功率He:Ne激光2周,15mW、25mW对术后早期(1个月)促进种植体周围成骨细胞增殖提高成骨细胞活性。Objective： To explore the best power of low-power laser irradiation on implant-osseointegration. Methods： 6 female Beagle dogs were selected according to international standards. Surgeries were operated on each dog from both sides of the tibia, four implant sockets were implanXed in 4 implants, every side 4 planting interval 2cm,which were divided into A, B, C ,D group randomly. D group which as control group was not irradiated. A （15roW）, B（25Mw）, C（35Mw） groups for the experimental groupwere immediately irradiated after surgery. The period of irradiation is two weeks , 20min/once, per day. Specimens were obtained 1, 2 and 3 months after implan- tation, from two animals at each time point. Before killing the animals tetracycline double labeling were exposed. Specimens were performed general observation and histology observation. Results： Bone histomorphometry analysis： one month after operation ,there was a statistically significant difference between A, B and C, D groups in min- eral apposition rate （MAR） and percent labeled perimeter（%L. Pm）. The MAR and ML. Pm of A, B groups was significantly higher than C, D groups. Two and three months after operation there was no statistically significant difference between four groups. H istomorphology analysis： on bone-implant contact（BIC） one and two months after operation there was no statistically significant difference between four groups. Three months after operation there was a statistically significant difference between A, B and C, D groups. The bone-implant contact （BIC） of A, B groups was significantly higher than C, D groups. Conclusions： Two weeks＇ irradiation of low-power laser play a significant promotion role in implant-osseointegration.

关 键 词：种植体低功率激光种植体-骨结合率动物研究 骨形态计量学 

分 类 号：R783.6[医药卫生—口腔医学]