离子液体强化选择性酶促合成异槲皮苷工艺优化  

Process Optimization of Selectively Enzymatic Synthesis of Isoquercitrin Using Ionic Liquid

作　　者：孙国霞[1] 王俊[1] 丁伟桐[1] 王凯旋[1] 吴福安[1,2] 

机构地区：[1]江苏科技大学生物与化学工程学院,镇江212018 [2]中国农业科学院蚕业研究所,镇江212018

出　　处：《中国生物工程杂志》2013年第3期130-134,共5页China Biotechnology

基　　金：国家自然科学基金(21206061);中国博士后基金面上项目(2012M510124);江苏省高校"青蓝工程"优秀青年骨干教师资助计划;江苏省科技支撑计划(农业)项目(BE2010419);江苏省自然科学基金面上项目(BK2009213);现代农业产业技术体系建设专项(CARS-22)

摘　　要：为了提高离子液体共溶剂效应强化选择性酶促合成异槲皮苷的得率,在前期单因素实验的基础上,利用响应曲面法对离子液体比例、酶浓度和底物浓度三因素进行优化。优化后的最佳工艺条件为:离子液体比例13.05%、酶浓度9.10 g/L、底物浓度0.72 g/L,优化后异槲皮苷的摩尔得率为99.27±0.55%,较优化前提高了8.60%。To improve the yield of enzymatic synthesis of isoquercitrin, the process condition of enzymatic reaction was optimized by response surface methodology. According to the results of single-factors experiments, response surface methodology was investigated the selected three factors： ionic liquid content, enzyme concentration and substrate concentration. The result showed that the optimum condition for the enzymatic synthesis of isoquercitrin using ionic liquid as a co-solvent was： ionic liquid content 13.05%, enzyme concentration 9.10 g/L, substrate concentration 0.72 g/L. Under the optimal condition, the yield of isoquercitrin was 99.27 ？ 0.55%, increased by 8.60% than before.

关 键 词：离子液体 共溶剂 响应曲面法 酶促合成 异槲皮苷 

分 类 号：Q815[生物学—生物工程]